Effect of Various Concentrations of Caffeine, Pentoxifylline, and Kallikrein on Hyperactivation of Frozen Bovine Semen

被引:35
作者
Barakat, Ibrahim A. H. [1 ,2 ]
Danfour, Mohamed A. [3 ]
Galewan, Fatma A. M. [3 ]
Dkhil, Mohamed A. [1 ,4 ]
机构
[1] King Saud Univ, Coll Sci, Dept Zool, Riyadh 11451, Saudi Arabia
[2] Natl Res Ctr, Dept Cell Biol, Giza 12622, Egypt
[3] Misurata Univ, Fac Med, Dept Physiol, Misurata, Libya
[4] Helwan Univ, Fac Sci, Dept Zool, Cairo 11795, Egypt
关键词
IN-VITRO FERTILIZATION; HUMAN SPERM MOTILITY; HUMAN SEMINAL PLASMA; HUMAN SPERMATOZOA; TISSUE KALLIKREIN; BULL SPERM; TNF-ALPHA; PENETRATION; IMPROVES; CA2+;
D O I
10.1155/2015/948575
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Caffeine, pentoxifylline, and kallikrein are substances that affect the efficiency of sperms in the fertilization process; however, they have not been adequately studied. The present study aimed to examine the influence of caffeine, kallikrein, and pentoxifylline on sperm motility in bovine as well as investigate their optimum concentrations for increasing the movement of sperms in bovine. Frozen bovine sperms were thawed in universal IVF medium supplemented with 1, 5, and 10 mM caffeine or pentoxifylline or 1, 4, and 8 U/mL kallikrein and were then incubated for 30 min. Treated semen parameters were analyzed using a computer assisted semen analyzer (CASA). Data analysis showed that the mean values concerning progression and motility of sperm increased in caffeine and pentoxifylline treatments when compared with the kallikrein group. The obtained results revealed that kallikrein is not necessary for the improvement of bovine sperm motility. Additionally, our results revealed that 5mM from caffeine was the best concentration added to the medium, followed by 1 or 5 mM from pentoxifylline. Therefore, it is concluded from the present study that caffeine has hyperactivation efficacy at 5 mM concentration compared to other treatments.
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页数:7
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