Influence of Extraction Protocol on Physical Properties of Parvovirus B19 DNA

被引：1
|
作者：
Matz, Bertfried [1 ]
Kupfer, Bernd [1 ]
Kreil, Thomas R. [2 ]
Eis-Huebinger, Anna Maria [1 ]
机构：
[1] Univ Bonn, Inst Virol, D-53105 Bonn, Germany
[2] Baxter, Vienna, Austria
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 12期
关键词：
CLONING; GENOME; VIRUS;
D O I：
10.1128/JCM.00571-10
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
While establishing a quantification standard for parvovirus B19 diagnostics, we extracted viral DNA from a high-titered human plasma by using three commercial kits. Despite similar viral DNA yields being obtained, striking differences in electrophoretic mobilities of the extracted nucleic acids were observed.
引用
收藏
页码：4595 / 4597
页数：3
相关论文
共 50 条
  • [1] ALBUMIN BATCHES AND B19 PARVOVIRUS DNA
    LEFRERE, JJ
    MARIOTTI, M
    DELACROIX, I
    LERABLE, J
    THAUVIN, M
    BURNOUF, T
    FOLLEA, G
    TRANSFUSION, 1995, 35 (05) : 389 - 391
  • [2] B19 PARVOVIRUS
    YOUNG, NS
    BAILLIERES CLINICAL HAEMATOLOGY, 1995, 8 (01): : 25 - 56
  • [3] Parvovirus B19
    Bültmann, BD
    Sotlar, K
    Klingel, K
    NEW ENGLAND JOURNAL OF MEDICINE, 2004, 350 (19): : 2006 - 2007
  • [4] Parvovirus B19
    Bundesgesundheitsblatt, 1998, 41 (2): : 83 - 87
  • [5] Parvovirus B19
    Burger, R
    Gerlich, W
    Gürtler, L
    Heiden, M
    Kretschmer, V
    Lefèvre, H
    Löwer, J
    Montag-Lessing, T
    Neumann, R
    Pauli, G
    Seitz, R
    Schlenkrich, U
    Werner, E
    Illkommen, HW
    TRANSFUSION MEDICINE AND HEMOTHERAPY, 2005, 32 (3-4) : 121 - 126
  • [6] Parvovirus B19
    Landry, Marie Louise
    MICROBIOLOGY SPECTRUM, 2016, 4 (03):
  • [7] Parvovirus B19
    Burger, R
    Gerlich, W
    Gürtler, L
    Heiden, M
    Kretschner, V
    Lefèvre, H
    Löwer, J
    Montag-Lessing, T
    Neumann, R
    Pauli, G
    Seitz, R
    Schlenkrich, U
    Werner, E
    Willkomen, H
    INFUSIONSTHERAPIE UND TRANSFUSIONSMEDIZIN, 1998, 25 : 97 - 102
  • [8] Enhanced kinetic extraction of parvovirus B19 structural proteins
    Sico, C
    White, S
    Tsao, E
    Varma, A
    BIOTECHNOLOGY AND BIOENGINEERING, 2002, 80 (03) : 250 - 256
  • [9] Detection of parvovirus B19 DNA in blood: Viruses or DNA remnants?
    Molenaar-de Backer, M. W. A.
    Russcher, A.
    Kroes, A. C. M.
    Koppelman, M. H. G. M.
    Lanfermeijer, M.
    Zaaijer, H. L.
    JOURNAL OF CLINICAL VIROLOGY, 2016, 84 : 19 - 23
  • [10] Parvovirus B19 infection
    Chen, S
    Howard, O
    NEW ENGLAND JOURNAL OF MEDICINE, 2004, 350 (06): : 598 - 598
12345