Mechanism of Differential Susceptibility of Two (Canine Lung Adenocarcinoma) Cell Lines to 5-Aminolevulinic Acid-Mediated Photodynamic Therapy

被引：2

作者：

Osaki, Tomohiro ^{[1
]}

Kunisue, Narumi ^{[2
]}

Ota, Urara ^{[2
]}

Imazato, Hideo ^{[2
]}

Ishii, Takuya ^{[2
]}

Takahashi, Kiwamu ^{[2
]}

Ishizuka, Masahiro ^{[2
]}

Tanaka, Tohru ^{[3
]}

Okamoto, Yoshiharu ^{[1
]}

机构：

[1] Tottori Univ, Fac Agr, Joint Dept Vet Clin Med, Tottori 6808553, Japan

[2] SBI Pharmaceut Co Ltd, Tokyo 1066020, Japan

[3] Neopharma Japan Co Ltd, Tokyo 1020071, Japan

来源：

CANCERS | 2021年 / 13卷 / 16期

关键词：

photodynamic therapy; lung adenocarcinoma; tumor; canine carcinoma; NITRIC-OXIDE; PROTOPORPHYRIN IX; CANCER; GLUTATHIONE; SENSITIZERS; ALA; RESISTANCE; EFFICIENCY; CARCINOMA; ROLES;

D O I：

10.3390/cancers13164174

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Photodynamic therapy (PDT) is a clinically approved, minimally invasive treatment for malignant tumors. Protoporphyrin IX (PpIX), derived from 5-aminolevulinic acid (5-ALA) as the prodrug, is one of the photosensitizers used in PDT. Recently, we reported a significant difference in response to 5-ALA-mediated PDT treatment in two canine primary lung adenocarcinoma cell lines (sensitive to PDT: HDC cells, resistant to PDT: LuBi cells). This study aimed to examine the difference in cytotoxicity of 5-ALA-mediated PDT in these cells. Although intracellular PpIX levels before irradiation were similar between HDC and LuBi cells, the percentage of ROS-positive cells and apoptotic cells in LuBi cells treated with 5-ALA-mediated PDT was significantly lower than that in HDC cells treated with 5-ALA-mediated PDT. A high dosage of the NO donor, DETA NONOate, significantly increased the cytotoxicity of 5-ALA-mediated PDT against LuBi cells. These results suggest that the sensitivity of 5-ALA-mediated PDT might be correlated with NO.

引用

页数：13

共 39 条

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