A simple and rapid method to assay triacylglycerol in cells and tissues

被引：76

作者：

Schwartz, Danielle M. ^{[1
]}

Wolins, Nathan E. ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Dept Med, Div Nutr Sci, St Louis, MO 63110 USA

来源：

JOURNAL OF LIPID RESEARCH | 2007年 / 48卷 / 11期

关键词：

fatty acids; fat; lipid; screen; acyl-glycerol;

D O I：

10.1194/jlr.D700017-JLR200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a reliable, rapid, and economical assay for the quantification of triacylglycerol (TG) in cells and animal tissues. In a few hours, this assay quantifies microgram amounts of TG from tens or even hundreds of samples. The protocol includes an organic extraction to partition TG away from proteins and other hydrophilic molecules found in cells and tissues that may interfere with the colorimetric enzyme-linked TG detection method. In addition, this assay is economical, as no expensive reagents, supplies, or equipment are needed. Another benefit of this assay is that it does not require environmentally unfriendly halogenated solvents.

引用

页码：2514 / 2520

页数：7

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