Determination of polycyclic aromatic hydrocarbons in food samples by automated on-line in-tube solid-phase microextraction coupled with high-performance liquid chromatography-fluorescence detection

被引:182
作者
Ishizaki, A. [1 ]
Saito, K. [1 ]
Hanioka, N. [2 ]
Narimatsu, S. [2 ]
Kataoka, H. [1 ]
机构
[1] Shujitsu Univ, Sch Pharm, Naka Ku, Okayama 7038516, Japan
[2] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Kita Ku, Okayama 7008530, Japan
关键词
Polycyclic aromatic hydrocarbons; In-tube solid-phase microextraction; Automated sample preparation; High-performance liquid chromatography-fluorescence detection; Food samples; SMOKED MEAT-PRODUCTS; MASS-SPECTROMETRY; RISK-ASSESSMENT; VEGETABLE-OILS; MILK SAMPLES; CANCER-RISK; OLIVE OILS; CONTAMINATION; EXPOSURE; SMOKING;
D O I
10.1016/j.chroma.2010.06.068
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple and sensitive automated method, consisting of in-tube solid-phase microextraction (SPME) coupled with high-performance liquid chromatography-fluorescence detection (HPLC-FLD), was developed for the determination of 15 polycyclic aromatic hydrocarbons (PAHs) in food samples. PAHs were separated within 15 min by HPLC using a Zorbax Eclipse PAH column with a water/acetonitrile gradient elution program as the mobile phase. The optimum in-tube SPME conditions were 20 draw/eject cycles of 40 mu L of sample using a CP-Sil 19CB capillary column as an extraction device. Low- and high-molecular weight PAHs were extracted effectively onto the capillary coating from 5% and 30% methanol solutions, respectively. The extracted PAHs were readily desorbed from the capillary by passage of the mobile phase, and no carryover was observed. Using the in-tube SPME HPLC-FLD method, good linearity of the calibration curve (r > 0.9972) was obtained in the concentration range of 0.05-2.0 ng/mL, and the detection limits (S/N = 3) of PAHs were 0.32-4.63 pg/mL. The in-tube SPME method showed 18-47 fold higher sensitivity than the direct injection method. The intra-day and inter-day precision (relative standard deviations) for a 1 ng/mL PAH mixture were below 5.1% and 7.6% (n = 5), respectively. This method was applied successfully to the analysis of tea products and dried food samples without interference peaks, and the recoveries of PAHs spiked into the tea samples were >70%. Low-molecular weight PAHs such as naphthalene and pyrene were detected in many foods, and carcinogenic benzo[a]pyrene, at relatively high concentrations, was also detected in some black tea samples. This method was also utilized to assess the release of PAHs from tea leaves into the liquor. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:5555 / 5563
页数:9
相关论文
共 37 条
[1]   A headspace solid-phase microextraction procedure coupled with gas chromatography-mass spectrometry for the analysis of volatile polycyclic aromatic hydrocarbons in milk samples [J].
Aguinaga, N. ;
Campillo, N. ;
Vinas, P. ;
Hernandez-Cordoba, M. .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2008, 391 (03) :753-758
[2]   Experimental design for the optimization of the extraction conditions of polycyclic aromatic hydrocarbons in milk with a novel diethoxydiphenylsilane solid-phase microextraction fiber [J].
Bianchi, F. ;
Careri, M. ;
Mangia, A. ;
Mattarozzi, M. ;
Musci, M. .
JOURNAL OF CHROMATOGRAPHY A, 2008, 1196 :41-45
[3]   Analysis of EU priority polycyclic aromatic hydrocarbons in food supplements using high performance liquid chromatography coupled to an ultraviolet, diode array or fluorescence detector [J].
Danyi, Sophie ;
Brose, Francois ;
Brasseur, Catherine ;
Schneider, Yves-Jacques ;
Larondelle, Yvan ;
Pussemier, Luc ;
Robbens, Johan ;
De Saeger, Sarah ;
Maghuin-Rogister, Guy ;
Scippo, Marie-Louise .
ANALYTICA CHIMICA ACTA, 2009, 633 (02) :293-299
[4]   Automated in-tube solid-phase microextraction coupled to high-performance liquid chromatography [J].
Eisert, R ;
Pawliszyn, J .
ANALYTICAL CHEMISTRY, 1997, 69 (16) :3140-3147
[5]   Polycyclic aromatic hydrocarbons in foods:: Human exposure through the diet in Catalonia, Spain [J].
Falcó, G ;
Domingo, JL ;
Llobet, JM ;
Teixidó, A ;
Casas, C ;
Müller, L .
JOURNAL OF FOOD PROTECTION, 2003, 66 (12) :2325-2331
[6]   Polycyclic aromatic hydrocarbons in food samples collected in Barcelona, Spain [J].
Fontcuberta, M. ;
Arques, J. F. ;
Martinez, M. ;
Suarez, A. ;
Villalbi, J. R. ;
Centrich, F. ;
Serrahima, E. ;
Duran, J. ;
Casas, C. .
JOURNAL OF FOOD PROTECTION, 2006, 69 (08) :2024-2028
[7]   Analysis of polycyclic aromatic hydrocarbons in vegetable oils combining gel permeation chromatography with solid-phase extraction clean-up [J].
Fromberg, A. ;
Hojgard, A. ;
Duedahl-Olesen, L. .
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2007, 24 (07) :758-767
[8]   Headspace solid-phase microextraction as a tool to estimate the contamination of smoked cheeses by polycyclic aromatic hydrocarbons [J].
Guillén, MD ;
Sopelana, P .
JOURNAL OF DAIRY SCIENCE, 2005, 88 (01) :13-20
[9]   Investigation of sample treatment steps for the analysis of polycyclic aromatic hydrocarbons in ground coffee [J].
Houessou, Justin Koffi ;
Delteil, Corine ;
Camel, Valerie .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (20) :7413-7421
[10]   Polycyclic aromatic hydrocarbons in fruits and vegetables grown in the Czech Republic [J].
Janska, M. ;
Hajslova, J. ;
Tomaniova, M. ;
Kocourek, V. ;
Vavrova, M. .
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 2006, 77 (04) :492-499