Microwave-mediated enzymatic modifications of DNA

被引：6

作者：

Das, Rakha Hari ^{[1
]}

Ahirwar, Rajesh ^{[1
]}

Kumar, Saroj ^{[1
]}

Nahar, Pradip ^{[1
]}

机构：

[1] CSIR, Inst Genom & Integrat Biol, Delhi 110007, India

来源：

ANALYTICAL BIOCHEMISTRY | 2015年 / 471卷

关键词：

Microwave; DNA; Digestion; Ligation; Dephosphorylation; Kination; IRRADIATION; SAMPLES;

D O I：

10.1016/j.ab.2014.11.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we report microwave-induced specific cleavage, ligation, dephosphorylation, and phosphorylation of nucleic acids catalyzed by restriction endonucleases, T4 DNA ligase, T4 polynucleotide kinase, and calf intestinal alkaline phosphatase. The microwave-mediated method has dramatically reduced the reaction time to 20 to 50 s. In control experiments, the same reactions failed to give the desired reaction products when carried out in the same time periods but without microwave irradiation. Because the microwave method is rapid, it could be a useful alternative to the time-consuming conventional procedure for enzymatic modification of DNA. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：26 / 28

页数：3

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