Oxidative stress-related gene polymorphism and the risk of preeclampsia

Objective: The purpose of this study was to determine whether genetic variability in oxidative stress-related enzymes contributes to individual preeclampsia susceptibility differences. Study Design: Polymorphisms in the cytochrome P450 (CYP)1A1 (Mspl), CKP1A1(Ile/Val), glutathione S-transferase (GS7-)M1, GSTT1, myeloperoxidase (MPO), and manganese superoxide dismutase (MnSOD) genes were evaluated by polymerase chain reaction (PCR) or PCR-restriction fragment length polymorphism (RFLP) in 214 healthy controls with an uncomplicated obstetric history, and in 121 preeclampsia patients. X analyses were used to statistically evaluate differences. Results: No significant differences in the CYP1A1(Mspl) or CYP1A1(Ile/Val) genotypes were observed between the healthy controls and the preeclampsia patients (X = 1.43, P = 0.49 versus X = 1.54, P = 0.46). The GSTM1 homozygous null type and GSTT1 homozygous null type were no differences in the patients and controls (X-2 = 0.01, P = 0.92 versus X-2 = 0.31, P = 0.57), and no significant differences in the polymorphisms of the MPO and MnSOD genotypes were found between the patients and controls (X-2 = 2.00, P = 0.37 versus X2 = 0.07, P = 0.96). Conclusion: Polymorphisms in the oxidative stress-related genes (CYP1A1, GSTM1, GSTT1, MPO, MnSOD) do not seem to be risk factors for preeclampsia. (c) 2004 Published by Elsevier Ireland Ltd.