Sensitization of cisplatin-resistant ovarian cancer cells by magnetite iron oxide nanoparticles: an in vitro study

被引：15

作者：

Gokduman, Kurtulus ^{[1
,2
]}

机构：

[1] Harvard Med Sch, Massachusetts Gen Hosp, Ctr Engn Med, Boston, MA 02114 USA

[2] Bogazici Univ, Inst Biomed Engn, TR-34684 Istanbul, Turkey

来源：

NANOMEDICINE | 2019年 / 14卷 / 24期

关键词：

caspase-3; cisplatin; Fe3O4; magnetite nanoparticles; MTT assay; ovarian cancer; OVCAR-3; SKOV-3; REDOX STATE UNBALANCE; ALPHA-LIPOIC ACID; OXIDATIVE STRESS; INDUCED NEPHROTOXICITY; ENERGETIC METABOLISM; FE3O4; NANOPARTICLES; MITOCHONDRIAL-DNA; DRUG-RESISTANCE; MECHANISMS; GLUTATHIONE;

D O I：

10.2217/nnm-2019-0126

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: To investigate potential of magnetite iron oxide nanoparticles (MION) to sensitize cisplatin-resistant ovarian cancer cells to cisplatin, which to the best of found knowledge has not been reported previously. Materials & methods: MION with a diameter of approximately 20 nm were synthesized, and characterized using Fourier transform infrared spectroscopy, powder x-ray diffraction and particle size analyzer. Results: The synthesized MION have increased reactive oxygen species levels and decreased glutathione levels in cisplatin-resistant ovarian cancer cells (OVCAR-3 and SKOV-3). Using MTT, capsase-3 activity and live/dead assays, capability of the synthesized MION to sensitize cisplatin-resistant ovarian cancer cells has been illustrated. Conclusion: Thus, for further investigations, the synthesized MION can be considered as a potent agent enabling much more effective cisplatin-based therapies for ovarian cancer.

引用

页码：3177 / 3191

页数：15

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