Inhibition of cell migration and invasion and promotion of cell apoptosis by overexpression of programmed cell death 4 (PDCD4) in cervical cancer Siha cells

Cervical cancer has been one of the leading causes of cancer-related deaths among women worldwide. However, few targeted drugs have been developed because of the poor understanding about the mechanisms of cervical cancer. A growing number of studies in recent years have shown that programmed cell death 4 (PDCD4), a new tumor suppressor, participates in the tumorigenesis and progression of various cancers. In this study, we investigated the influence of PDCD4 on cell migration, invasion, and apoptosis of cervical cancer cells. Siha cervical cancer cells were transfected with a recombinant lentivirus vector carrying the complete length of the PDCD4 gene and the normal controlled vector respectively and screened by puromycin. The expression of the mRNA and protein of PDCD4 were significantly elevated in Siha cells transfected with the recombinant vector carrying the PDCD4 gene. Then the overexpression of PDCD4 suppressed the cell migration and invasion in transwell migration and matrigel invasion assays respectively. Moreover, the overexpression of PDCD4 increased the proportion of cell apoptosis in flow cytometry analysis. In a multiple signal pathways assay, the upregulation of PDCD4 promoted the phosphorylation of some key proteins, such as p53 and STAT1. These results suggest that PDCD4 is a potential therapeutic target for cervical cancer.