Detection and Sequence Analysis of Equine Gammaherpesviruses from Horses with Respiratory Tract Disease in Turkey

被引：33
作者：
Ataseven, V. S. [1 ]
Bilge-Dagalp, S. [2 ]
Oguzoglu, T. C. [2 ]
Karapinar, Z. [3 ]
Guzel, M. [4 ]
Tan, M. T. [5 ]
机构：
[1] Mustafa Kemal Univ, Dept Virol, Fac Vet Med, TR-31040 Antakya, Hatay, Turkey
[2] Ankara Univ, Dept Virol, Fac Vet Med, TR-06100 Ankara, Turkey
[3] Yuzuncu Yil Univ, Dept Virol, Fac Vet Med, Van, Turkey
[4] Mustafa Kemal Univ, Dept Internal Med, Fac Vet Med, TR-31040 Antakya, Hatay, Turkey
[5] Adnan Menderes Univ, Dept Virol, Fac Vet Med, Aydin, Turkey
来源：
TRANSBOUNDARY AND EMERGING DISEASES | 2010年 / 57卷 / 04期
关键词：
equid herpesvirus 2; equid herpesvirus 5; Mn-PCR; respiratory disease; sequencing; Turkey; HERPESVIRUS TYPE-2 EHV-2; PREVALENCE; INFECTIONS; FOALS; IDENTIFICATION;
D O I：
10.1111/j.1865-1682.2010.01146.x
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
P>The equid herpesvirus 2 (EHV-2) and 5 (EHV-5), identified agents of respiratory infections and keratoconjunctivitis cases in some equids, comprise a high degree of antigenic heterogeneity. Prevalence and genetic characterization of EHV-2 and EHV-5 strains from Turkey were investigated in this study. A total of 73 nasal swabs and 54 blood specimens were sampled from horses with respiratory tract diseases characterized by mucopurulent nasal discharge and occasional coughing. Overall, EHV-2- and EHV-5-specific DNA amplicons were obtained from 19.2% (14/73) and 21.9% (16/73) of horses tested by multiplex nested PCR. Sequences of EHV-2 and EHV-5 glycoprotein B (gB) gene were used in a phylogenetic analysis that included six EHV-2 and three EHV-5 isolates, which showed that the Turkish EHV-2 and EHV-5 strains have marked sequence divergence from European strains and from each other. Turkish EHV-2 isolates were divided into two distinct subdivisions, and a few isolates were located on a separate branch. This study provides the first epidemiological and phylogenetical report about EHV-2 and EHV-5 infections in Turkey.
引用
收藏
页码：271 / 276
页数：6
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