Phytase production by Bacillus subtilis US417 in submerged and solid state fermentations

被引：25

作者：

Kammoun, Radhouane ^{[1
]}

Farhat, Ameny ^{[1
]}

Chouayekh, Hichem ^{[1
]}

Bouchaala, Kameleddine ^{[1
]}

Bejar, Samir ^{[1
]}

机构：

[1] Univ Sfax, Lab Microorganismes & Biomol, Ctr Biotechnol Sfax, Sfax 3018, Tunisia

来源：

ANNALS OF MICROBIOLOGY | 2012年 / 62卷 / 01期

关键词：

Phytase; Response surface methodology; Submerged fermentation; Solid state fermentation; Bacillus subtilis; Modelling; RESPONSE-SURFACE METHODOLOGY; THERMOSTABLE PHYTASE; STATISTICAL OPTIMIZATION; CULTURE-CONDITIONS; ESCHERICHIA-COLI; WHEAT BRAN; PURIFICATION; EXPRESSION; GENE; OILCAKES;

D O I：

10.1007/s13213-011-0240-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

When the variables (inoculum size, methanol and yeast extract) identified to affect phytase production by Bacillus subtilis US417 using Plackett-Burman design were optimized by RSM, a high enzyme production of 112 U/g of wheat bran was attained. Overall, a 5-fold improvement in phytase production was achieved. In SSF, on the other hand, a 4-fold enhancement in enzyme titer was attained (85 U/g of wheat bran). Based on these findings, phytase productivity was higher in SF [2.3 U/(g x h)] than in SSF [1.2 U/(g x h)].

引用

页码：155 / 164

页数：10

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