Light quality affects flavonoid biosynthesis in young berries of Cabernet Sauvignon grape

被引：217
作者：
Koyama, Kazuya [1 ]
Ikeda, Hiroko [1 ]
Poudel, Puspa Raj [1 ]
Goto-Yamamoto, Nami [1 ]
机构：
[1] Natl Res Inst Brewing, Hiroshima 7390046, Japan
来源：
PHYTOCHEMISTRY | 2012年 / 78卷
关键词：
Red-wine grape; Vitis vinifera; Vitaceae; Ultraviolet; Visible light; Photoprotection; Secondary metabolism; MYB transcription factor superfamily; Flavonoid; Proanthocyanidin; Flavo nol; R2R3-MYB TRANSCRIPTION FACTOR; REGULATES PROANTHOCYANIDIN SYNTHESIS; SYNTHASE GENE-EXPRESSION; UV-B RADIATION; ANTHOCYANIN BIOSYNTHESIS; PHENYLPROPANOID BIOSYNTHESIS; ULTRAVIOLET-RADIATION; PLANT HORMONES; SKIN; ACCUMULATION;
D O I：
10.1016/j.phytochem.2012.02.026
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Biosynthesis of phenolic compounds is known to be sensitive to light environments, which reflects the possible role of these compounds for photoprotection in plants. Herein, the effects of UV and visible light on biosynthesis of flavonoids was investigated, i.e., proanthocyanidins (PAs) and flavonols, in young berry skins of a red-wine grape. Vitis vinifera cv. Cabernet Sauvignon. Shading with light-proof boxes from the flowering stage until 49 days after treatment (DAT) partially decreased PA concentrations, and completely decreased flavonol concentrations in the berry skins. Shading decreased the transcript abundance of a flavonol-related gene more remarkably than those of PA-related genes. In addition, light exclusion influenced the composition of PAs, such as the decrease in the proportion of trihydroxylated subunits and the mean degree of polymerization (mDP) within PM. However, solar UV exclusion did not affect the concentration and composition of PM, whereas this exclusion remarkably decreased the flavonol concentration. Consistently, UV exclusion did not influence the transcript levels of PA-related genes, whereas it dramatically decreased that of flavonol-related genes. These findings indicated a different light regulation of the biosynthesis of these flavonoids in young berry skins of wine grape. Visible light primarily induces biosynthesis of PAs and affects their composition, whereas UV light specifically induces biosynthesis of flavonols. Distinct roles of members of a MYB transcription factor family for light regulation of flavonoid biosynthesis were proposed. (C) 2012 Elsevier Ltd. All rights reserved.
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页码：54 / 64
页数：11
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