Bacterial CYP153A monooxygenases for the synthesis of omega-hydroxylated fatty acids

被引：87

作者：

Malca, Sumire Honda ^{[1
]}

Scheps, Daniel ^{[1
]}

Kuehnel, Lisa ^{[1
]}

Venegas-Venegas, Elena ^{[1
]}

Seifert, Alexander ^{[1
]}

Nestl, Bettina M. ^{[1
]}

Hauer, Bernhard ^{[1
]}

机构：

[1] Univ Stuttgart, Inst Tech Biochem, D-70569 Stuttgart, Germany

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 42期

关键词：

TERMINAL-ALKANE HYDROXYLATION; YARROWIA-LIPOLYTICA; N-ALKANES; CYTOCHROME-P450; POLYMERS; GENES; P450;

D O I：

10.1039/c2cc18103g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

CYP153A from Marinobacter aquaeolei has been identified as a fatty acid omega-hydroxylase with a broad substrate range. Two hotspots predicted to influence substrate specificity and selectivity were exchanged. Mutant G307A is 2- to 20-fold more active towards fatty acids than the wild-type. Residue L354 is determinant for the enzyme omega-regioselectivity.

引用

收藏

页码：5115 / 5117

页数：3

相关论文

共 35 条

[11] Biotechnological synthesis of long-chain dicarboxylic acids as building blocks for polymers [J].

Huf, Sabine ;

Kruegener, Sven ;

Hirth, Thomas ;

Rupp, Steffen ;

Zibek, Susanne .

EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, 2011, 113 (05) :548-561

[12] Biochemical and structural characterization of CYP124: A methyl-branched lipid ω-hydroxylase from Mycobacterium tuberculosis [J].

Johnston, Jonathan B. ;

Kells, Petrea M. ;

Podust, Larissa M. ;

de Montellano, Paul R. Ortiz .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (49) :20687-20692

[13] Isolation and functional analysis of cytochrome p450 CYP153A genes from various environments [J].

Kubota, M ;

Nodate, M ;

Yasumoto-Hirose, M ;

Uchiyama, T ;

Kagami, O ;

Shizuri, Y ;

Misawa, N .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2005, 69 (12) :2421-2430

[14] Altering the regioselectivity of cytochrome P450CYP102A3 of Bacillus subtilis by using a new versatile assay system [J].

Lentz, O ;

Feenstra, A ;

Habicher, T ;

Hauer, B ;

Schmid, RD ;

Urlacher, VB .

CHEMBIOCHEM, 2006, 7 (02) :345-350

[15] Polymers from Fatty Acids: Poly(ω-hydroxyl tetradecanoic acid) Synthesis and Physico-Mechanical Studies [J].

Liu, Chen ;

Liu, Fei ;

Cai, Jiali ;

Xie, Wenchun ;

Long, Timothy E. ;

Turner, S. Richard ;

Lyons, Alan ;

Gross, Richard A. .

BIOMACROMOLECULES, 2011, 12 (09) :3291-3298

[16] Biosynthesis of Monomers for Plastics from Renewable Oils [J].

Lu, Wenhua ;

Ness, Jon E. ;

Xie, Wenchun ;

Zhang, Xiaoyan ;

Minshull, Jeremy ;

Gross, Richard A. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (43) :15451-15455

[17] Molecular characterization of the 56-kDa CYP153 from Acinetobacter sp EB104 [J].

Maier, T ;

Förster, HH ;

Asperger, O ;

Hahn, U .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 286 (03) :652-658

[18] Engineering cytochrome P450BM3 for terminal alkane hydroxylation [J].

Meinhold, P ;

Peters, MW ;

Hartwick, A ;

Hernandez, AR ;

Arnold, FH .

ADVANCED SYNTHESIS & CATALYSIS, 2006, 348 (06) :763-772

[19] Structure conservation in cytochromes P450 [J].

Mestres, J .

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 2005, 58 (03) :596-609

[20]

Oriedo J., 2003, WO Pat., Patent No. [2003/020017, 2003020017]

← 1 2 3 4 →