Bacterial CYP153A monooxygenases for the synthesis of omega-hydroxylated fatty acids

被引：87

作者：

Malca, Sumire Honda ^{[1
]}

Scheps, Daniel ^{[1
]}

Kuehnel, Lisa ^{[1
]}

Venegas-Venegas, Elena ^{[1
]}

Seifert, Alexander ^{[1
]}

Nestl, Bettina M. ^{[1
]}

Hauer, Bernhard ^{[1
]}

机构：

[1] Univ Stuttgart, Inst Tech Biochem, D-70569 Stuttgart, Germany

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 42期

关键词：

TERMINAL-ALKANE HYDROXYLATION; YARROWIA-LIPOLYTICA; N-ALKANES; CYTOCHROME-P450; POLYMERS; GENES; P450;

D O I：

10.1039/c2cc18103g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

CYP153A from Marinobacter aquaeolei has been identified as a fatty acid omega-hydroxylase with a broad substrate range. Two hotspots predicted to influence substrate specificity and selectivity were exchanged. Mutant G307A is 2- to 20-fold more active towards fatty acids than the wild-type. Residue L354 is determinant for the enzyme omega-regioselectivity.

引用

页码：5115 / 5117

页数：3

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