Quince (Cydonia oblonga Miller) peel polyphenols modulate LPS-induced inflammation in human THP-1-derived macrophages through NF-κB, p38MAPK and Akt inhibition

被引:72
作者
Essafi-Benkhadir, Khadija [2 ]
Refai, Amira [1 ]
Riahi, Ichrak [2 ]
Fattouch, Sami [3 ]
Karoui, Habib [2 ]
Essafi, Makram [1 ]
机构
[1] Inst Pasteur Tunis, Lab Rech Transmiss Controle & Immunobiol Infect, Tunis 1002, Tunisia
[2] Inst Pasteur Tunis, Lab Epidemiol Mol & Pathol Expt Appl Malad Infect, Tunis 1002, Tunisia
[3] Natl Inst Appl Sci & Technol, Lab LIP MB, Tunis, Tunisia
关键词
Quince; Polyphenols; Macrophages; Inflammation; Cytokines; Signalling pathways; ACTIVATED PROTEIN-KINASE; LIPOPOLYSACCHARIDE; INTERLEUKIN-6; EXPRESSION; CELLS; TNF; ARTHRITIS; ENDOTOXIN; FRESH; MODEL;
D O I
10.1016/j.bbrc.2012.01.003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chronic inflammation is a hallmark of several pathologies, such as rheumatoid arthritis, gastritis, inflammatory bowel disease, atherosclerosis and cancer. A wide range of anti-inflammatory chemicals have been used to treat such diseases while presenting high toxicity and numerous side effects. Here, we report the anti-inflammatory effect of a non-toxic, cost-effective natural agent, polyphenolic extract from the Tunisian quince Cydonia oblonga Miller. Lipopolysaccharide (LPS) treatment of human THP-1-derived macrophages induced the secretion of high levels of the pro-inflammatory cytokine TNF-alpha and the chemokine IL-8, which was inhibited by quince peel polyphenolic extract in a dose-dependent manner. Concomitantly, quince polyphenols enhanced the level of the anti-inflammatory cytokine IL-10 secreted by LPS-treated macrophages. We further demonstrated that the unexpected increase in IL-6 secretion that occurred when quince polyphenols were associated with LPS treatment was partially responsible for the polyphenols-mediated inhibition of TNF-alpha secretion. Biochemical analysis showed that quince polyphenols extract inhibited the LPS-mediated activation of three major cellular pro-inflammatory effectors, nuclear factor-kappa B (NF-kappa B), p38MAPIC and Akt. Overall, our data indicate that quince peel polyphenolic extract induces a potent anti-inflammatory effect that may prove useful for the treatment of inflammatory diseases and that a quince-rich regimen may help to prevent and improve the treatment of such diseases. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码:180 / 185
页数:6
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