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Quince (Cydonia oblonga Miller) peel polyphenols modulate LPS-induced inflammation in human THP-1-derived macrophages through NF-κB, p38MAPK and Akt inhibition
被引:72
作者:
Essafi-Benkhadir, Khadija
[2
]
Refai, Amira
[1
]
Riahi, Ichrak
[2
]
Fattouch, Sami
[3
]
Karoui, Habib
[2
]
Essafi, Makram
[1
]
机构:
[1] Inst Pasteur Tunis, Lab Rech Transmiss Controle & Immunobiol Infect, Tunis 1002, Tunisia
[2] Inst Pasteur Tunis, Lab Epidemiol Mol & Pathol Expt Appl Malad Infect, Tunis 1002, Tunisia
[3] Natl Inst Appl Sci & Technol, Lab LIP MB, Tunis, Tunisia
关键词:
Quince;
Polyphenols;
Macrophages;
Inflammation;
Cytokines;
Signalling pathways;
ACTIVATED PROTEIN-KINASE;
LIPOPOLYSACCHARIDE;
INTERLEUKIN-6;
EXPRESSION;
CELLS;
TNF;
ARTHRITIS;
ENDOTOXIN;
FRESH;
MODEL;
D O I:
10.1016/j.bbrc.2012.01.003
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Chronic inflammation is a hallmark of several pathologies, such as rheumatoid arthritis, gastritis, inflammatory bowel disease, atherosclerosis and cancer. A wide range of anti-inflammatory chemicals have been used to treat such diseases while presenting high toxicity and numerous side effects. Here, we report the anti-inflammatory effect of a non-toxic, cost-effective natural agent, polyphenolic extract from the Tunisian quince Cydonia oblonga Miller. Lipopolysaccharide (LPS) treatment of human THP-1-derived macrophages induced the secretion of high levels of the pro-inflammatory cytokine TNF-alpha and the chemokine IL-8, which was inhibited by quince peel polyphenolic extract in a dose-dependent manner. Concomitantly, quince polyphenols enhanced the level of the anti-inflammatory cytokine IL-10 secreted by LPS-treated macrophages. We further demonstrated that the unexpected increase in IL-6 secretion that occurred when quince polyphenols were associated with LPS treatment was partially responsible for the polyphenols-mediated inhibition of TNF-alpha secretion. Biochemical analysis showed that quince polyphenols extract inhibited the LPS-mediated activation of three major cellular pro-inflammatory effectors, nuclear factor-kappa B (NF-kappa B), p38MAPIC and Akt. Overall, our data indicate that quince peel polyphenolic extract induces a potent anti-inflammatory effect that may prove useful for the treatment of inflammatory diseases and that a quince-rich regimen may help to prevent and improve the treatment of such diseases. (C) 2012 Elsevier Inc. All rights reserved.
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页码:180 / 185
页数:6
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