A qPCR MGB probe based eDNA assay for European freshwater pearl mussel (Margaritifera margaritifera L.)

被引：17

作者：

Carlsson, Jeanette E. L. ^{[1
]}

Egan, Damian ^{[2
]}

Collins, Patrick C. ^{[1
,3
]}

Farrell, Edward D. ^{[1
]}

Igoe, Fran ^{[4
]}

Carlsson, Jens ^{[1
]}

机构：

[1] Univ Coll Dublin, Sch Biol & Environm Sci, Earth Inst, Dublin, Ireland

[2] Univ Coll Dublin, Sch Biol & Environm Sci, Dublin, Ireland

[3] Queens Univ, Marine Lab QML, Portaferry, Down, North Ireland

[4] 2 Boherduff Fethard Rd, Clonmel, Ireland

来源：

AQUATIC CONSERVATION-MARINE AND FRESHWATER ECOSYSTEMS | 2017年 / 27卷 / 06期

基金：

爱尔兰科学基金会;

关键词：

conservation; detection; environmental DNA; non-invasive; monitoring; quantitative PCR; ENVIRONMENTAL DNA; SAMPLES;

D O I：

10.1002/aqc.2788

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

1. Environmental (e)DNA assays are becoming increasingly used to detect rare or invasive aquatic species. 2. The Critically Endangered freshwater pearl mussel Margaritifera margaritifera is undergoing range-wide reduction in population numbers and distribution. 3. An eDNA assay to detect the presence of M. margaritifera was developed, based on the mitochondrial cytochrome oxidase I gene, utilizing species-specific primers, a minor groove binding (MGB) probe and quantitative (q)PCR approaches. 4. The results from this pilot study demonstrated high sensitivity both in laboratory and field trials, and provide a valuable non-invasive tool for detecting M. margaritifera.

引用

页码：1341 / 1344

页数：4

共 18 条

[1] Anon, 2004, IR WILDL MAN, V12
[2] VARIATION IN THE LIFE-SPAN AND SIZE OF THE FRESH-WATER PEARL MUSSEL
BAUER, G
[J]. JOURNAL OF ANIMAL ECOLOGY, 1992, 61 (02) : 425 - 436
[3] THE STATUS OF THE FRESH-WATER PEARL MUSSEL MARGARITIFERA-MARGARITIFERA L IN THE SOUTH OF ITS EUROPEAN RANGE
BAUER, G
[J]. BIOLOGICAL CONSERVATION, 1986, 38 (01) : 1 - 9
[4] Improved detection of an alien invasive species through environmental DNA barcoding: the example of the American bullfrog Lithobates catesbeianus
Dejean, Tony
Valentini, Alice
Miquel, Christian
Taberlet, Pierre
Bellemain, Eva
Miaud, Claude
[J]. JOURNAL OF APPLIED ECOLOGY, 2012, 49 (04) : 953 - 959
[5] Species detection using environmental DNA from water samples
Ficetola, Gentile Francesco
Miaud, Claude
Pompanon, Francois
Taberlet, Pierre
[J]. BIOLOGY LETTERS, 2008, 4 (04) : 423 - 425
[6] Strategies for the conservation of endangered freshwater pearl mussels (Margaritifera margaritifera L.): a synthesis of Conservation Genetics and Ecology
Geist, Juergen
[J]. HYDROBIOLOGIA, 2010, 644 (01) : 69 - 88
[7] Molecular Detection of Vertebrates in Stream Water: A Demonstration Using Rocky Mountain Tailed Frogs and Idaho Giant Salamanders
Goldberg, Caren S.
Pilliod, David S.
Arkle, Robert S.
Waits, Lisette P.
[J]. PLOS ONE, 2011, 6 (07):
[8] An eDNA assay for Irish Petromyzon marinus and Salmo trutta and field validation in running water
Gustavson, M. S.
Collins, P. C.
Finarelli, J. A.
Egan, D.
Conchuir, R. O.
Wightman, G. D.
King, J. J.
Gauthier, D. T.
Whelan, K.
Carlsson, J. E. L.
Carlsson, J.
[J]. JOURNAL OF FISH BIOLOGY, 2015, 87 (05) : 1254 - 1262
[9] "Sight-unseen" detection of rare aquatic species using environmental DNA
Jerde, Christopher L.
Mahon, Andrew R.
Chadderton, W. Lindsay
Lodge, David M.
[J]. CONSERVATION LETTERS, 2011, 4 (02): : 150 - 157
[10] 3′-minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures
Kutyavin, IV
Afonina, IA
Mills, A
Gorn, VV
Lukhtanov, EA
Belousov, ES
Singer, MJ
Walburger, DK
Lokhov, SG
Gall, AA
Dempcy, R
Reed, MW
Meyer, RB
Hedgpeth, J
[J]. NUCLEIC ACIDS RESEARCH, 2000, 28 (02) : 655 - 661

← 1 2 →