A novel polysaccharide from Castanea mollissima Blume: Preparation, characteristics and antitumor activities in vitro and in vivo

被引：54
作者：
Dong, Xiao-dan [1 ,2 ]
Feng, Ying-ying [1 ,2 ]
Liu, Yi-ning [1 ,2 ]
Ji, Hai-yu [1 ,2 ]
Yu, Sha-sha [1 ,2 ]
Liu, Anjun [1 ]
Yu, Juan [1 ]
机构：
[1] Tianjin Univ Sci & Technol, Coll Food Engn & Biotechnol, Tianjin 300457, Peoples R China
[2] QingYunTang Biotech Beijing Co Ltd, Beijing Econ Technol Dev Area, 14 Zhonghe St, Beijing 100176, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2020年 / 240卷
关键词：
Castanea mollissima Blume; Polysaccharide; Characteristics; Antitumor; SARGASSUM-FUSIFORME POLYSACCHARIDES; STRUCTURAL-CHARACTERIZATION; ANTIOXIDANT; CELLS; APOPTOSIS;
D O I：
10.1016/j.carbpol.2020.116323
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A new water-soluble polysaccharide, CMP90, with a molecular weight of 23.9 kDa was isolated from Castanea mollissima Blume and the preliminary structural characteristics and antitumor effects of CMP90 in vitro and in vivo were investigated in the research. CMP90 consists of arabinose, galactose, glucose, xylose and mannose (molar ratio: 0.08:0.11:5.14:0.12:0.08) with alpha- and beta-anomeric units. The results of in vitro experiments indicated that CMP90 exhibited a significant inhibitory effect on the proliferation of HL-60 cells with typical apoptotic characteristics by inducing cell cycle arrested at G1/M phase. Additionally, the results in vivo suggested CMP90 was able to inhibit the growth of S180 solid tumors via protecting immune organs, improving the levels of serum cytokines (TNF-alpha, IL-2 and IFN-gamma), enhancing the activities of immune cells (macrophages, lymphocytes and NK cells) and inducing cell apoptosis or death. Taken together, these combined data clearly indicated that CMP90 may be used as a potential candidate agent for cancer therapy.
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页数：9
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