Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations

被引：1380

作者：

Kalyanaraman, Balaraman ^{[1
,2
]}

Darley-Usmar, Victor ^{[3
,4
]}

Davies, Kelvin J. A. ^{[5
,6
]}

Dennery, Phyllis A. ^{[7
]}

Forman, Henry Jay ^{[5
,8
]}

Grisham, Matthew B. ^{[9
,10
]}

Mann, Giovanni E. ^{[11
]}

Moore, Kevin ^{[12
]}

Roberts, L. Jackson, II ^{[13
]}

Ischiropoulos, Harry ^{[7
]}

机构：

[1] Med Coll Wisconsin, Dept Biophys, Milwaukee, WI 53226 USA

[2] Med Coll Wisconsin, Free Rad Res Ctr, Milwaukee, WI 53226 USA

[3] Univ Alabama Birmingham, Ctr Free Rad Biol, Birmingham, AL 35294 USA

[4] Univ Alabama Birmingham, Dept Pathol, Birmingham, AL 35294 USA

[5] Univ So Calif, Ethel Percy Andrus Gerontol Ctr, Davis Sch Gerontol, Dornsife Coll Letters Arts & Sci, Los Angeles, CA 90089 USA

[6] Univ So Calif, Dornsife Coll Letters Arts & Sci, Div Mol & Computat Biol, Los Angeles, CA 90089 USA

[7] Univ Penn, Dept Pediat, Div Neonatol, Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA

[8] Univ Calif Merced, Merced, CA 95344 USA

[9] Louisiana State Univ, Hlth Sci Ctr, Immunol & Inflammat Res Grp, Shreveport, LA 71130 USA

[10] Louisiana State Univ, Hlth Sci Ctr, Dept Mol & Cellular Physiol, Shreveport, LA 71130 USA

[11] Kings Coll London, Div Cardiovasc, BHF Ctr Res Excellence, London WC2R 2LS, England

[12] UCL, Sch Med, Dept Med, London W1N 8AA, England

[13] Vanderbilt Univ, Dept Pharmacol, Nashville, TN 37240 USA

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 2012年 / 52卷 / 01期

基金：

英国医学研究理事会;

关键词：

ENDOTHELIAL-CELLS; OXIDATIVE STRESS; NITRIC-OXIDE; INTRACELLULAR SUPEROXIDE; MEDIATED OXIDATION; CYTOCHROME-C; PEROXYNITRITE; HYDROETHIDINE; PRODUCT; INDICATOR;

D O I：

10.1016/j.freeradbiomed.2011.09.030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The purpose of this position paper is to present a critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species. Where feasible, we have made recommendations for the use of alternate probes and appropriate analytical techniques that measure the specific products formed from the reactions between fluorescent probes and reactive oxygen and nitrogen species. We have proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：1 / 6

页数：6

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