Materials fabrication from Bombyx mori silk fibroin

被引:2385
作者
Rockwood, Danielle N. [1 ]
Preda, Rucsanda C. [1 ]
Yucel, Tuna [1 ]
Wang, Xiaoqin [1 ]
Lovett, Michael L. [1 ]
Kaplan, David L. [1 ]
机构
[1] Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
MESENCHYMAL STEM-CELLS; ENGINEERED VASCULAR GRAFTS; IN-VITRO; POLY(ETHYLENE OXIDE); ADENOSINE RELEASE; 3-D SCAFFOLDS; 3D SCAFFOLDS; BONE-MARROW; BIOMATERIALS; POLYMER;
D O I
10.1038/nprot.2011.379
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Silk fibroin, derived from Bombyx mori cocoons, is a widely used and studied protein polymer for biomaterial applications. Silk fibroin has remarkable mechanical properties when formed into different materials, demonstrates biocompatibility, has controllable degradation rates from hours to years and can be chemically modified to alter surface properties or to immobilize growth factors. A variety of aqueous or organic solvent-processing methods can be used to generate silk biomaterials for a range of applications. In this protocol, we include methods to extract silk from B. mori cocoons to fabricate hydrogels, tubes, sponges, composites, fibers, microspheres and thin films. These materials can be used directly as biomaterials for implants, as scaffolding in tissue engineering and in vitro disease models, as well as for drug delivery.
引用
收藏
页码:1612 / 1631
页数:20
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