An Expanded Palette of Genetically Encoded Ca2+ Indicators

被引：983

作者：

Zhao, Yongxin ^{[1
]}

Araki, Satoko ^{[2
]}

Jiahui, Wu ^{[1
]}

Teramoto, Takayuki ^{[3
]}

Chang, Yu-Fen ^{[2
]}

Nakano, Masahiro ^{[2
]}

Abdelfattah, Ahmed S. ^{[1
]}

Fujiwara, Manabi ^{[3
]}

Ishihara, Takeshi ^{[3
]}

Nagai, Takeharu ^{[2
,4
]}

Campbell, Robert E. ^{[1
]}

机构：

[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada

[2] Hokkaido Univ, Res Inst Elect Sci, Kita Ku, Sapporo, Hokkaido 0010020, Japan

[3] Kyushu Univ, Fac Sci, Dept Biol, Higashi Ku, Fukuoka 8128581, Japan

[4] Japan Sci & Technol Agcy, PRESTO, Chiyoda Ku, Tokyo 1020075, Japan

来源：

SCIENCE | 2011年 / 333卷 / 6051期

基金：

日本学术振兴会; 加拿大健康研究院; 日本科学技术振兴机构; 加拿大自然科学与工程研究理事会;

关键词：

GREEN FLUORESCENT PROTEINS; GCAMP2;

D O I：

10.1126/science.1208592

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Engineered fluorescent protein (FP) chimeras that modulate their fluorescence in response to changes in calcium ion (Ca2+) concentration are powerful tools for visualizing intracellular signaling activity. However, despite a decade of availability, the palette of single FP-based Ca2+ indicators has remained limited to a single green hue. We have expanded this palette by developing blue, improved green, and red intensiometric indicators, as well as an emission ratiometric indicator with an 11,000% ratio change. This series enables improved single-color Ca2+ imaging in neurons and transgenic Caenorhabditis elegans. In HeLa cells, Ca2+ was imaged in three subcellular compartments, and, in conjunction with a cyan FP-yellow FP-based indicator, Ca2+ and adenosine 5'-triphosphate were simultaneously imaged. This palette of indicators paints the way to a colorful new era of Ca2+ imaging.

引用

页码：1888 / 1891

页数：4

共 15 条

[1] Crystal Structures of the GCaMP Calcium Sensor Reveal the Mechanism of Fluorescence Signal Change and Aid Rational Design [J].

Akerboom, Jasper ;

Rivera, Jonathan D. Velez ;

Guilbe, Maria M. Rodriguez ;

Malave, Elisa C. Alfaro ;

Hernandez, Hector H. ;

Tian, Lin ;

Hires, S. Andrew ;

Marvin, Jonathan S. ;

Looger, Loren L. ;

Schreiter, Eric R. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (10) :6455-6464

[2] Quantitative export of a reporter protein, GFP, by the twin-arginine translocation pathway in Escherichia coli [J].

Barrett, CML ;

Ray, N ;

Thomas, JD ;

Robinson, C ;

Bolhuis, A .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 304 (02) :279-284

[3] Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators [J].

Imamura, Hiromi ;

Nhat, Kim P. Huynh ;

Togawa, Hiroko ;

Saito, Kenta ;

Iino, Ryota ;

Kato-Yamada, Yasuyuki ;

Nagai, Takeharu ;

Noji, Hiroyuki .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (37) :15651-15656

[4] Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin [J].

Miyawaki, A ;

Llopis, J ;

Heim, R ;

McCaffery, JM ;

Adams, JA ;

Ikura, M ;

Tsien, RY .

NATURE, 1997, 388 (6645) :882-887

[5] Circularly permuted green fluorescent proteins engineered to sense Ca2+ [J].

Nagai, T ;

Sawano, A ;

Park, ES ;

Miyawaki, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (06) :3197-3202

[6] A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein [J].

Nakai, J ;

Ohkura, M ;

Imoto, K .

NATURE BIOTECHNOLOGY, 2001, 19 (02) :137-141

[7] Ca2+ Regulation of Mitochondrial ATP Synthesis Visualized at the Single Cell Level [J].

Nakano, Masahiro ;

Imamura, Hiromi ;

Nagai, Takeharu ;

Noji, Hiroyuki .

ACS CHEMICAL BIOLOGY, 2011, 6 (07) :709-715

[8] Improving the photostability of bright monomeric orange and red fluorescent proteins [J].

Shaner, Nathan C. ;

Lin, Michael Z. ;

McKeown, Michael R. ;

Steinbach, Paul A. ;

Hazelwood, Kristin L. ;

Davidson, Michael W. ;

Tsien, Roger Y. .

NATURE METHODS, 2008, 5 (06) :545-551

[9] Behavioral Choice between Conflicting Alternatives Is Regulated by a Receptor Guanylyl Cyclase, GCY-28, and a Receptor Tyrosine Kinase, SCD-2, in AIA Interneurons of Caenorhabditis elegans [J].

Shinkai, Yoichi ;

Yamamoto, Yuta ;

Fujiwara, Manabi ;

Tabata, Takashi ;

Murayama, Takashi ;

Hirotsu, Takaaki ;

Ikeda, Daisuke D. ;

Tsunozaki, Makoto ;

Iino, Yuichi ;

Bargmann, Cornelia I. ;

Katsura, Isao ;

Ishihara, Takeshi .

JOURNAL OF NEUROSCIENCE, 2011, 31 (08) :3007-3015

[10] Imaging cellular signals in the heart in vivo:: Cardiac expression of the high-signal Ca2+ indicator GCaMP2 [J].

Tallini, YN ;

Ohkura, M ;

Choi, BR ;

Ji, GJ ;

Imoto, K ;

Doran, R ;

Lee, J ;

Plan, P ;

Wilson, J ;

Xin, HB ;

Sanbe, A ;

Gulick, J ;

Mathai, J ;

Robbins, J ;

Salama, G ;

Nakai, J ;

Kotlikoff, MI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (12) :4753-4758

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