DNA looping mediates nucleosome transfer

被引:27
作者
Brennan, Lucy D. [1 ]
Forties, Robert A. [1 ,2 ,4 ]
Patel, Smita S. [3 ]
Wang, Michelle D. [1 ,2 ]
机构
[1] Cornell Univ, Dept Phys, Lab Atom & Solid State Phys, Ithaca, NY 14853 USA
[2] Cornell Univ, Howard Hughes Med Inst, Ithaca, NY 14853 USA
[3] Rutgers Robert Wood Johnson Med Sch, Dept Biochem & Mol Biol, Piscataway, NJ 08854 USA
[4] Advion Inc, 30 Brown Rd, Ithaca, NY 14850 USA
来源
NATURE COMMUNICATIONS | 2016年 / 7卷
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
RNA-POLYMERASE-II; REPLICATION FORK; SINGLE-MOLECULE; H2A/H2B DIMER; HIGH-AFFINITY; T7; HELICASE; TRANSCRIPTION; DYNAMICS; STABILITY; CHAINS;
D O I
10.1038/ncomms13337
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Proper cell function requires preservation of the spatial organization of chromatin modifications. Maintenance of this epigenetic landscape necessitates the transfer of parental nucleosomes to newly replicated DNA, a process that is stringently regulated and intrinsically linked to replication fork dynamics. This creates a formidable setting from which to isolate the central mechanism of transfer. Here we utilized a minimal experimental system to track the fate of a single nucleosome following its displacement, and examined whether DNA mechanics itself, in the absence of any chaperones or assembly factors, may serve as a platform for the transfer process. We found that the nucleosome is passively transferred to available dsDNA as predicted by a simple physical model of DNA loop formation. These results demonstrate a fundamental role for DNA mechanics in mediating nucleosome transfer and preserving epigenetic integrity during replication.
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页数:8
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