Hybridization biosensor using sodium tanshinone IIA sulfonate as electrochemical indicator for detection of short DNA species of chronic myelogenous leukemia

被引:17
作者
Chen, Jinghua [1 ]
Zhang, Jing [2 ]
Zhuang, Qian [1 ]
Chen, Jing [1 ]
Lin, Xinhua [1 ]
机构
[1] Fujan Med Univ, Dept Pharmaceut Anal, Fac Pharm, Fuzhou 350004, Peoples R China
[2] Fujan Coll Med Occupat & Technol, Dept Pharmaceut, Fuzhou 350101, Peoples R China
基金
中国国家自然科学基金;
关键词
sodium tanshinone IIA sulfonate; hybridization biosensor; intercalative binding; electrochemical indicator; chronic myelogenous leukemia;
D O I
10.1016/j.electacta.2007.08.059
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
Cyclic voltammetry (CV) was used to investigate electrochemical behavior of sodium tanshinone IIA sulfonate (STS) and the interaction between STS and salmon sperm DNA. STS had excellent electrochemical activity on the glassy carbon electrode (GCE) with a couple reversible redox peaks. In pH 4.0 phosphate buffer solution (PBS), the binding ratio between STS and salmon sperm DNA was calculated to be 1:1 and the binding constant was 1.67 x 10(4) L/mol. A chronic myelogenous leukemia (CML, Type b3a2) DNA biosensor was developed by immobilizing covalently single-stranded CIVIL DNA fragment to a modified GCE. The surface hybridization of the immobilized single-stranded CML DNA fragment with its complementary DNA fragment was evidenced by electrochemical methods using STS as a novel electrochemical indicator, with a detection limit of 6.7 x 10(-9) M and a linear range from 2.0 x 10(-8) M to 2.0 x 10(-7) M. Selective determination of complementary ssDNA was achieved using differential pulse voltammetry (DPV). (c) 2007 Published by Elsevier Ltd.
引用
收藏
页码:2716 / 2723
页数:8
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