Application of flow cytometry of yeast in research and practice

被引:0
|
作者
Novak, Jan [1 ]
Basarova, Gabriela [1 ]
Fiala, Jaromir [1 ]
Dostalek, Pavel [1 ]
机构
[1] Inst Chem Technol, Dept Fermentat Chem & Bioengn, CR-16628 Prague 6, Czech Republic
来源
CHEMICKE LISTY | 2008年 / 102卷 / 03期
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中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Flow cytometry as a rapid technique can be used for monitoring the yeast (Saccharomyces cerevisiae) growth in biotechnological processes. The technique permits analysis of single cells in suspension and thus to obtain cell size distribution, granularity, intracellular contents of DNA, proteins, and sterols, viability, cell cycle, intracellular pH and enzyme activities. Flow cytometry can also be used for cell sorting. Despite the advantages of flow cytometry, implementation of the technique in biotechnological laboratories is still limited.
引用
收藏
页码:183 / 187
页数:5
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