Single muscle fiber proteomics reveals unexpected mitochondrial specialization

被引：146

作者：

Murgia, Marta ^{[1
,2
]}

Nagaraj, Nagarjuna ^{[1
]}

Deshmukh, Atul S. ^{[1
,3
]}

Zeiler, Marlis ^{[1
]}

Cancellara, Pasqua ^{[2
]}

Moretti, Irene ^{[4
]}

Reggiani, Carlo ^{[2
]}

Schiaffino, Stefano ^{[4
]}

Mann, Matthias ^{[1
,3
]}

机构：

[1] Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

[2] Univ Padua, Dept Biomed Sci, Padua, Italy

[3] Univ Copenhagen, Fac Hlth Sci, Novo Nordisk Fdn Ctr Prot Res, Dept Prote, Copenhagen, Denmark

[4] Venetian Inst Mol Med, Padua, Italy

来源：

EMBO REPORTS | 2015年 / 16卷 / 03期

关键词：

exercise; metabolism; mitochondria; muscle fibers; single cell; SKELETAL-MUSCLE; PROTEIN-COMPOSITION; IDENTIFICATION; EXPRESSION; DEHYDROGENASE; METABOLISM; DIVERSITY; PATTERNS; HEALTH; DRIVES;

D O I：

10.15252/embr.201439757

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here, we developed a high-sensitivity work-flow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and unbiased proteomics methods yielded the same subtype assignment. We discovered novel subtype-specific features, most prominently mitochondrial specialization of fiber types in substrate utilization. The fiber type-resolved proteomes can be applied to a variety of physiological and pathological conditions and illustrate the utility of single cell type analysis for dissecting proteomic heterogeneity.

引用

页码：387 / 395

页数：9

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