Cytotoxicity and cellular uptake of lysozyme-stabilized gold nanoparticles

被引：18

作者：

Lee, Yeonju ^{[1
]}

Geckeler, Kurt E. ^{[1
,2
,3
]}

机构：

[1] GIST, Dept Mat Sci & Engn, Kwangju 500712, South Korea

[2] WCU, Dept Nanobio Mat & Elect, GIST, Kwangju 500712, South Korea

[3] GIST, Inst Med Syst Engn, Kwangju 500712, South Korea

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2012年 / 100A卷 / 04期

关键词：

cytotoxicity; cellular uptake; gold nanoparticles; lysozyme; microwave; CONTROLLED BULK SYNTHESIS; IN-SITU SYNTHESIS; CARBON NANOTUBES; PROTEIN; NANOMATERIALS; CHEMISTRY; TOXICITY;

D O I：

10.1002/jbm.a.34020

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The particle size and surface properties of gold nanoparticles are critical factors for the interactions between nanoparticles and cells. To produce noncytotoxic gold nanoparticles, a straightforward method for the synthesis of gold nanoparticles designed involving the reduction and stabilization by a protein such as a lysozyme in conjunction with microwave irradiation. The cooperative combination of a lysozyme with a high affinity for metal ions and the microwave irradiation allowed to form biocompatible gold nanoparticles in an aqueous system. In addition, the cell toxicity and the cellular uptake pathways of the gold nanoparticles synthesized against mouse embryonic fibroblast NIH-3T3 cells were studied and found to be taken up by receptor-mediated endocytosis. In addition, the lysozyme-stabilized gold nanoparticles are accumulated in the cytoplasm as well as the nucleus without any significant cytotoxicity. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.

引用

页码：848 / 855

页数：8

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