Development and evaluation of dual-target real-time polymerase chain reaction assays to detect Bordetella spp.

被引:50
|
作者
Tatti, Kathleen M. [1 ]
Wu, Kai-Hui [1 ]
Tondella, Maria Lucia [1 ]
Cassiday, Pamela K. [1 ]
Cortese, Margaret M. [1 ]
Wilkins, Patricia P. [1 ]
Sanden, Gary N. [1 ]
机构
[1] Ctr Dis Control & Prevent, Natl Ctr Immunizat & Resp Dis, Div Bacterial Dis, Meningitis & Vaccine Preventable Dis Branch, Atlanta, GA 30333 USA
关键词
Bordetella pertussis; real-time PCR; IS481; pertussis toxin gene;
D O I
10.1016/j.diagmicrobio.2008.02.017
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Novel, highly specific, and sensitive real-time polymerase chain reaction (PCR) assays using 2 targets, insertion sequence (IS481) and pertussis toxin subunit 1 (ptxS1), were developed to detect Bordetella pertussis and to differentiate between relevant Bordetella spp. Sixty-four non-Bordetella isolates were negative by both assays, demonstrating the specificity of the assays. B. pertussis, Bordetella parapertussis, and Bordetella holmesii isolates were specifically identified using the assays. The lower limit of detection was less than 10 genomic equivalents per reaction for the IS481 and ptxS1 assays. These assays were evaluated using 145 human clinical specimens obtained during cough-illness outbreak investigations, and PCR results were compared with Bordetella spp. culture results. Twenty-seven (18.6%) specimens had late positive cycle threshold (Ct) values (35 <= Ct<40) using the IS481 assay with corresponding negative results using the ptxS1 assay and culture and were considered indeterminate. Guidelines for use of PCR testing and interpretation of results during cough-illness outbreaks are discussed. Published by Elsevier Inc.
引用
收藏
页码:264 / 272
页数:9
相关论文
共 50 条
  • [1] Comparative evaluation of three real-time polymerase chain reaction assays to detect Myxobolus cerebralis
    Cavender, Wade
    Swan, Christine
    Wolf, Skylar
    Van Vliet, Danielle
    Johnson, Alison Aceves
    Forest, Anna
    Shields, Robert
    Loch, Thomas
    Knupp, Christopher
    Drennan, John
    Glenney, Gavin
    Hallett, Sascha L.
    Marcino, Joe
    Reed, Aimee
    JOURNAL OF AQUATIC ANIMAL HEALTH, 2024, 36 (03) : 250 - 264
  • [2] Evaluation of two real-time polymerase chain reaction pathogen detection kits for Salmonella spp. in food
    Cheung, PY
    Chan, CW
    Wong, W
    Cheung, TL
    Kam, KM
    LETTERS IN APPLIED MICROBIOLOGY, 2004, 39 (06) : 509 - 515
  • [3] Evaluation of amplification targets for the specific detection of Bordetella pertussis using real-time polymerase chain reaction
    Hasan, Mohammad Rubayet
    Tan, Rusung
    Al-Rawahi, Ghada N.
    Thomas, Eva
    Tilley, Peter
    CANADIAN JOURNAL OF INFECTIOUS DISEASES & MEDICAL MICROBIOLOGY, 2014, 25 (04): : 217 - 221
  • [4] Detection and identification of Candida spp. in human serum by LightCycler® real-time polymerase chain reaction
    Dunyach, Catherine
    Bertout, Sebastien
    Phelipeau, Cecile
    Drakulovski, Pascal
    Reynes, Jacques
    Mallie, Michele
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2008, 60 (03) : 263 - 271
  • [5] Development of a Novel Real-Time Polymerase Chain Reaction Assay to Detect Escherichia albertii in Chicken Meat
    Arai, Sakura
    Ooka, Tadasuke
    Shibata, Mizuha
    Nagai, Yuhki
    Tokoi, Yuki
    Nagaoka, Hiromi
    Maeda, Rika
    Tsuchiya, Akihiko
    Kojima, Yuka
    Ohya, Kenji
    Ohnishi, Takahiro
    Konishi, Noriko
    Ohtsuka, Kayoko
    Hara-Kudo, Yukiko
    FOODBORNE PATHOGENS AND DISEASE, 2022, 19 (12) : 823 - 829
  • [6] Evaluation of a Real time polymerase chain reaction assay for the detection of aflatoxin / sterigmatocystin producing strains of Aspergillus spp.
    Singh, R.
    Rai, T. S.
    Sharma, N. S.
    Arora, A. K.
    Kaur, Paviter
    INDIAN JOURNAL OF ANIMAL RESEARCH, 2017, 51 (04) : 676 - 678
  • [7] Real-time polymerase chain reaction detection of Bordetella pertussis DNA in acellular pertussis vaccines
    Tatti, Kathleen M.
    Slade, Barbara
    Patel, Manisha
    Messonnier, Nancy
    Jackson, Tiffany
    Kirkland, Kathryn B.
    Talbot, Elizabeth A.
    Tondella, M. Lucia
    PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2008, 27 (01) : 73 - 74
  • [8] Real-time polymerase chain reaction assays for the detection of members of the Mycoplasma mycoides cluster
    Fitzmaurice, J.
    Sewell, M.
    Manso-Silvan, L.
    Thiaucourt, F.
    McDonald, W. L.
    O'Keefe, J. S.
    NEW ZEALAND VETERINARY JOURNAL, 2008, 56 (01) : 40 - 47
  • [9] Validation and application of real-time polymerase chain reaction assays for representative rumen bacteria
    Koike, Satoshi
    Yabuki, Hiroyoshi
    Kobayashi, Yasuo
    ANIMAL SCIENCE JOURNAL, 2007, 78 (02) : 135 - 141
  • [10] Efficiency of real-time polymerase chain reaction assay to detect Vibrio vulnificus in seawater
    Kamio, Akira
    Hara-Kudo, Yukiko
    Miyasaka, Jiro
    Yahiro, Shunsuke
    Konuma, Hirotaka
    INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH, 2008, 211 (5-6) : 518 - 523