NDM-producing Enterobacteriaceae in a Chinese hospital, 2014-2015: identification of NDM-producing Citrobacter werkmanii and acquisition of blaNDM-1-carrying plasmid in vivo in a clinical Escherichia coli isolate

被引:36
作者
An, Jingna [1 ,2 ]
Guo, Ling [1 ]
Zhou, Lin [1 ]
Ma, Yanning [1 ]
Luo, Yanping [1 ]
Tao, Chuanmin [2 ]
Yang, Jiyong [1 ]
机构
[1] Chinese Peoples Liberat Army Gen Hosp, Dept Microbiol, Beijing, Peoples R China
[2] Sichuan Univ, West China Hosp, Dept Lab Med, Chengdu, Peoples R China
关键词
KLEBSIELLA-PNEUMONIAE; CARBAPENEM RESISTANCE; EMERGENCE; OUTBREAK; DISSEMINATION; SPREAD; STRAINS; FAMILY; ST37;
D O I
10.1099/jmm.0.000357
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
New Delhi metallo-beta-lactamase (NDM)-producing Enterobacteriaceae (NPE) shows prevalence in China. Little is known about the mechanisms related to the spread of NPE. Recently, a total of 51 non-duplicated NPE isolates were collected from a tertiary-care hospital in China and analysed for genetic relatedness by PFGE, antimicrobial susceptibility by Etest and sequence type by multilocus sequence typing. S1-PFGE and Southern blot analysis or PCR amplification were used for plasmid profiling. Between 2014 and 2015, 22 Escherichia coli, 10 Klebsiella pneumoniae, 9 Enterobacter cloacae, 2 Enterobacter aerogenes, 3 Providencia rettgeri, 1 Klebsiella oxytoca, 1 Proteus mirabilis, 1 Citrobacter freundii, 1 Citrobacter werkmanii and 1 Raoultella planticola were identified as NPE. Results of PFGE and multilocus sequence typing showed that most strains were genetically unrelated. Among the 45 bla(NDM)-carrying plasmids, there were 25 IncX3 plasmids with a size of about 30 to 50 kb, one 100 kb IncX3 plasmid, 11 IncA/C plasmids with a size range from 70 to 300 kb, six 90 to 120 kb IncB/O plasmids, one IncN plasmid with a size of 100 kb and one 140 kb IncFrep plasmid. An NDM-1-producing isolate of C. werkmanii was identified, which had not been reported previously. An Escherichia coli strain was found acquiring a bla(NDM-1)-carrying IncFrep plasmid in vivo during infection. In conclusion, an NDM-1-producing isolate of C. werkmanii was identified. An Escherichia coli strain acquired a bla(NDM-1)-carrying plasmid in vivo. IncX3 and IncA/C plasmids with various sizes might have emerged as the main platforms mediating the spread of the bla(NDM) genes in China.
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收藏
页码:1253 / 1259
页数:7
相关论文
共 30 条
[1]  
[Anonymous], 2013, MOL CELLULAR BIOCH
[2]   Infection by and dissemination of NDM-5-producing Escherichia coli in China [J].
Chen, Dongke ;
Gong, Lin ;
Walsh, Timothy R. ;
Lan, Ruiting ;
Wang, Tuo ;
Zhang, Jidong ;
Mai, Wenhui ;
Ni, Na ;
Lu, Jinxing ;
Xu, Jianguo ;
Li, Juan .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2016, 71 (02) :563-565
[3]   Unique genes identified in the epidemic extremely drug-resistant KPC-producing Klebsiella pneumoniae sequence type 258 [J].
Chmelnitsky, I. ;
Shklyar, M. ;
Hermesh, O. ;
Navon-Venezia, S. ;
Edgar, R. ;
Carmeli, Y. .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2013, 68 (01) :74-83
[4]  
Clinical and Laboratory Standards Institute (CLSI), 2014, PERF STAND ANT SUSC, V34
[5]   Identification and characterization of a novel incompatibility group X3 plasmid carrying blaNDM-1 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China [J].
Ho, Pak-Leung ;
Li, Zhen ;
Lo, Wai-U ;
Cheung, Yuk-Yam ;
Lin, Chi-Ho ;
Sham, Pak-Chung ;
Cheng, Vincent Chi-Chung ;
Ng, Tak-Keung ;
Que, Tak-Lun ;
Chow, Kin-Hung .
EMERGING MICROBES & INFECTIONS, 2012, 1
[6]   Emergence of blaNDM-1 among Klebsiella pneumoniae ST15 and novel ST1031 clinical isolates in China [J].
Hu, Longhua ;
Zhong, Qiaoshi ;
Tu, Jinjing ;
Xu, Yuanyuan ;
Qin, Zhiqiang ;
Parsons, Chris ;
Zhang, Bailing ;
Hu, Xiaoyan ;
Wang, Liangxing ;
Yu, Fangyou ;
Pan, Jingye .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2013, 75 (04) :373-376
[7]   Outbreak of Multidrug Resistant NDM-1-Producing Klebsiella pneumoniae from a Neonatal Unit in Shandong Province, China [J].
Jin, Yan ;
Shao, Chunhong ;
Li, Jian ;
Fan, Hui ;
Bai, Yuanyuan ;
Wang, Yong .
PLOS ONE, 2015, 10 (03)
[8]   Plasmid replicon typing of commensal and pathogenic Escherichia coli isolates [J].
Johnson, Timothy J. ;
Wannemuehler, Yvonne M. ;
Johnson, Sara J. ;
Logue, Catherine M. ;
White, David G. ;
Doetkott, Curt ;
Nolan, Lisa K. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (06) :1976-1983
[9]   Expansion of the IncX plasmid family for improved identification and typing of novel plasmids in drug-resistant Enterobacteriaceae [J].
Johnson, Timothy J. ;
Bielak, Eliza M. ;
Fortini, Daniela ;
Hansen, Lars Hestbjerg ;
Hasman, Henrik ;
Debroy, Chitrita ;
Nolan, Lisa K. ;
Carattoli, Alessandra .
PLASMID, 2012, 68 (01) :43-50
[10]   Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study [J].
Kumarasamy, Karthikeyan K. ;
Toleman, Mark A. ;
Walsh, Timothy R. ;
Bagaria, Jay ;
Butt, Fafhana ;
Balakrishnan, Ravikumar ;
Chaudhary, Uma ;
Doumith, Michel ;
Giske, Christian G. ;
Irfan, Seema ;
Krishnan, Padma ;
Kumar, Anil V. ;
Maharjan, Sunil ;
Mushtaq, Shazad ;
Noorie, Tabassum ;
Paterson, David L. ;
Pearson, Andrew ;
Perry, Claire ;
Pike, Rachel ;
Rao, Bhargavi ;
Ray, Ujjwayini ;
Sarma, Jayanta B. ;
Sharma, Madhu ;
Sheridan, Elizabeth ;
Thirunarayan, Mandayam A. ;
Turton, Jane ;
Upadhyay, Supriya ;
Warner, Marina ;
Welfare, William ;
Livermore, David M. ;
Woodford, Neil .
LANCET INFECTIOUS DISEASES, 2010, 10 (09) :597-602