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Adaptation of cotton shoot apex culture to Agrobacterium-mediated transformation
被引：50
作者：
Gould, JH
Magallanes-Cedeno, M
机构：
[1] Texas A&M Univ, Dept Forest Sci, College Stn, TX 77843 USA
[2] Univ Nevada, Dept Biochem, Reno, NV 89557 USA
关键词：
Agrobacterium;
cotton;
Gossypium;
shoot apex;
transformation;
D O I：
10.1023/A:1007438104369
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ;
081704 ;
摘要：
A protocol is presented for rapid genotype-independent transformation and regeneration of cotton (Gossypium spp.) from shoots isolated from germinating seedlings. Isolated shoots are inoculated with a super-virulent strain of Agrobacterium tumefaciens, subjected to a mild antibiotic selection, and directly regenerated as shoots in vitro. Shoots do not dedifferentiate and mutation rates are low. Rooted shoots can be obtained within 6–10 weeks of isolation and inoculation depending on the cotton cultivar.
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页码：283 / 283
页数：1
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