Human monocyte-derived dendritic cells produce bioactive gelatinase B:: Inhibition by IFN-ß

被引：45

作者：

Bartholomé, EJ

Van Aelst, I

Koyen, E

Kiss, R

Willems, F

Goldman, M

Opdenakker, G

机构：

[1] Free Univ Brussels, Hop Erasme, Dept Immunol, B-1070 Brussels, Belgium

[2] Free Univ Brussels, Ctr Rech Interuniv Vaccinol, B-1070 Brussels, Belgium

[3] Free Univ Brussels, Histol Lab, B-1070 Brussels, Belgium

[4] Katholieke Univ Leuven, Rega Inst Med Res, Lab Mol Immunol, B-3000 Louvain, Belgium

来源：

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH | 2001年 / 21卷 / 07期

关键词：

D O I：

10.1089/10799900152434367

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We studied the secretion of gelatinase B by dendritic cells (DC) generated by culturing human peripheral blood monocytes in granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). First, we found the intracellular expression of gelatinase B on sections of fixed DC pellets. Zymography analysis of the supernatants of DC cultured for 72 h demonstrated the presence of gelatinase B. To determine if DC produce net enzymatic activity, bioactive gelatinase, a novel sensitive fluorescent-activated substrate conversion (FASC) assay was used to complement the zymography data. Culture media of unstimulated DC demonstrated reproducible net gelatinolytic activity. Tumor necrosis factor-alpha (TNF-alpha) IL-1 beta but not lipopolysaccharide (LPS) stimulation caused a significant increase in gelatinase B production in zymography analysis. Both types of stimulation failed to increase net gelatinase activity in FASC assay. Interestingly, interferon-beta (IFN-beta) significantly diminished both the total zymolytic production and the net bioactive gelatinase produced by DC in a dose-dependent manner. We conclude that human monocyte-derived DC secrete bioactive gelatinase B and that IFN-beta inhibits this production.

引用

页码：495 / 501

页数：7

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