Folding transition into a loosely collapsed state in plasmid DNA as revealed by single-molecule observation

被引：20

作者：

Sato, YT

Hamada, T

Kubo, K

Yamada, A

Kishida, T

Mazda, O

Yoshikawa, K ^{[1
]}

机构：

[1] Kyoto Univ, Dept Phys, Grad Sch Sci, Kyoto 6068502, Japan

[2] Nagoya Univ, Grad Sch Environm Studies, Nagoya, Aichi 4648601, Japan

[3] Kyoto Prefectural Univ Med, Dept Microbiol, Kyoto 6028566, Japan

[4] Louis Pasteur Ctr Med Res, Kyoto 6068225, Japan

来源：

FEBS LETTERS | 2005年 / 579卷 / 14期

基金：

日本学术振兴会;

关键词：

circular DNA; DNA condensation; single DNA observation; colloidal particles; DNA assembly; spermine; DNA morphology;

D O I：

10.1016/j.febslet.2005.04.072

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conformational transition of a plasmid DNA, pGEG.GL3 (12.5 kbp, circular), induced by spermine(4+) was studied through the observation of individual DNA by fluorescence microscopy. We deduced the change in the hydrodynamic radius RH from an analysis of the Brownian motion of single DNA molecules. RH decreases in a continuous manner with an increase in spermine(4+), in contrast to the large discrete on/off change for long linear DNA. Just after the transition to the collapsed state, a small number of DNA molecules tend to form an assembly, which disperses in the bulk solution without precipitation. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3095 / 3099

页数：5

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