S-adenosyl-L-methionine-dependent O-methylation of 2-hydroxy-3-alkylpyrazine in wine grapes:: A putative final step of methoxypyrazine biosynthesis

被引：32

作者：

Hashizume, K ^{[1
]}

Tozawa, K ^{[1
]}

Endo, M ^{[1
]}

Aramaki, I ^{[1
]}

机构：

[1] Natl Res Inst Brewing, Higashihiroshima 7390046, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2001年 / 65卷 / 04期

关键词：

methoxypyrazine; methyltransferase; wine rape; hydroxypyrazine;

D O I：

10.1271/bbb.65.795

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The final step of 2-methoxy-3-alkylpyrazine (MP) biosynthesis has been presumed to involve O-methylation of 2-hydroxy-3-alklylpyarzine (HB), although this reaction has never been demonstrated in organisms. We detected 2-hydroxy-3-isobutylpyrazine (IBHP) and ? 2-hydroxy-3-isopropylpyrazine (IPHP) in unripe grapes, and S-adenosyl-L-methionine-dependent O-methyltransferase (OMT) activity toward HP in crude e?;tracts from young shoots and unripe grapes that accumulate II-IF at different levels, The levels of HP in the berries :Ind stems were estimated by using 2-hydroxy-3-sec-butylpyrazine as an internal standard. The OMT activity observed in the crude extracts from young shoot and berries was extremely low, but no MP-decomposing activity was detected in the solutions. The Levels of HP and OMT activity were closely related with the level of MP in the grapes. These results indicate that the predicted final step of MP biosynthesis exists in wine grapes.

引用

页码：795 / 801

页数：7

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