N-DEVD-N′-morpholinecarbonyl-rhodamine 110:: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay

被引：42

作者：

Wang, ZQ ^{[1
]}

Liao, JF ^{[1
]}

Diwu, ZJ ^{[1
]}

机构：

[1] Mol Devices Corp, Dept Reagent & Assay Dev, Sunnyvale, CA 94089 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2005年 / 15卷 / 09期

关键词：

fluorescent; fluorogenic substrates; caspase; apoptosis; synthesis; HTS;

D O I：

10.1016/j.bmcl.2005.02.081

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A novel caspase-3 substrate N-Ac-DEVD-N'-MC-R110, which is a fluorogenic substrate cleavable in a single step, has been prepared. It has a significantly higher enzyme turnover rate and sensitivity for detecting caspase-3 activity both in solution and living cells than existing fluorogenic substrates. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：2335 / 2338

页数：4

共 12 条

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