Potentiometric biosensors for cholinesterase inhibitor analysis based on mediatorless bioelectrocatalysis

被引：37

作者：

Ghindilis, AL ^{[1
]}

Morzunova, TG ^{[1
]}

Barmin, AV ^{[1
]}

Kurochkin, IN ^{[1
]}

机构：

[1] RES CTR MOL DIAGNOST & THERAPY,DEPT BIOSENSORS,MOSCOW 113149,RUSSIA

来源：

BIOSENSORS & BIOELECTRONICS | 1996年 / 11卷 / 09期

关键词：

mediatorless bioelectrocatalysis; butyrylcholinesterase; choline oxidase; peroxidase; inhibitor cholinesterase; biosensor;

D O I：

10.1016/0956-5663(96)89436-X

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A potentiometric method for cholinesterase inhibitor analysis based on mediatorless bioelectrocatalysis has been developed. The method includes coimmobilization of three enzymes, butyrylcholinesterase, choline oxidase and peroxidase, on composite carbon electrodes. Catalytic hydrolysis of butyrylcholine and subsequent catalytic oxidation of choline result in the formation of hydrogen peroxide leads to a shift in the electrode potential. The detection limit for trichlorfon analysis is 2 x 10(-13) M. Electrodes remain stable for at least 4 weeks when stored at 277 K. (C) 1996 Elsevier Science Limited.

引用

页码：873 / 880

页数：8

共 21 条

[1] DETERMINATION OF BUTYRYLCHOLINESTERASE INHIBITION USING ION TRANSFER ACROSS THE INTERFACE BETWEEN 2 IMMISCIBLE LIQUIDS [J].

BEATTIE, PD ;

INFELTA, PP ;

GIRAULT, HH .

ANALYTICAL CHEMISTRY, 1994, 66 (01) :52-57

[2] ANTICHOLINESTERASE ACTIVITY MEASUREMENT BY A CHOLINE BIOSENSOR - APPLICATION IN WATER ANALYSIS [J].

BERNABEI, M ;

CHIAVARINI, S ;

CREMISINI, C ;

PALLESCHI, G .

BIOSENSORS & BIOELECTRONICS, 1993, 8 (05) :265-271

[3] AN ENZYME AMPEROMETRIC SENSOR FOR TOXICANT DETERMINATION [J].

BUDNIKOV, HC ;

MEDYANTSEVA, EP ;

BABKINA, SS .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1991, 310 (1-2) :49-55

[4] BUTYRYLCHOLINE ENZYME SENSOR FOR DETERMINING ORGANOPHOSPHORUS INHIBITORS [J].

CAMPANELLA, L ;

ACHILLI, M ;

SAMMARTINO, MP ;

TOMASSETTI, M .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1991, 26 (02) :237-249

[5] AMPEROMETRIC SENSORS FOR PEROXIDE, CHOLINE, AND ACETYLCHOLINE BASED ON ELECTRON-TRANSFER BETWEEN HORSERADISH-PEROXIDASE AND A REDOX POLYMER [J].

GARGUILO, MG ;

HUYNH, N ;

PROCTOR, A ;

MICHAEL, AC .

ANALYTICAL CHEMISTRY, 1993, 65 (05) :523-528

[6] GLUCOSE POTENTIOMETRIC ELECTRODES BASED ON MEDIATORLESS BIOELECTROCATALYSIS - A NEW APPROACH [J].

GHINDILIS, AL ;

KUROCHKIN, IN .

BIOSENSORS & BIOELECTRONICS, 1994, 9 (4-5) :353-357

[7] SENSITIVE DETECTION OF PESTICIDES USING A DIFFERENTIAL ISFET-BASED SYSTEM WITH IMMOBILIZED CHOLINESTERASES [J].

HENDJI, AMN ;

JAFFREZICRENAULT, N ;

MARTELET, C ;

CLECHET, P ;

SHULGA, AA ;

STRIKHA, VI ;

NETCHIPORUK, LI ;

SOLDATKIN, AP ;

WLODARSKI, WB .

ANALYTICA CHIMICA ACTA, 1993, 281 (01) :3-11

[8] DETERMINATION OF CHOLINESTERASE IN BLOOD-SERUM WITH A BENZOATE-SENSITIVE MEMBRANE-ELECTRODE [J].

KATSU, T ;

KAYAMOTO, T .

ANALYTICA CHIMICA ACTA, 1991, 254 (1-2) :95-97

[9] BIENZYME SENSORS BASED ON CHEMICALLY MODIFIED ELECTRODES [J].

KULYS, J ;

SCHMID, RD .

BIOSENSORS & BIOELECTRONICS, 1991, 6 (01) :43-48

[10] AMPEROMETRIC ACETYLCHOLINE AND CHOLINE SENSORS WITH IMMOBILIZED ENZYMES [J].

MASCINI, M ;

MOSCONE, D .

ANALYTICA CHIMICA ACTA, 1986, 179 :439-444

← 1 2 3 →