Development and Analysis of a Collagen-Based Hemostatic Adhesive

Background Commercially available hemostatic adhesive has the risk of disease transmission because it is derived from a plasma component The purpose of this study is to manufacture a new hemostatic adhesive and evaluate its performances Methods Two atelocollagen based hemostatic adhesives were produced and named as Bleestop A (2% esterified atelocollagen in 75% ethanol + 1% CaCl2 + 0 71% DOPA + 0 1% tranexamic acid in DW) and Bleestop B (2% esterified atelocollagen in DW + 1% CaCl2 + 0 71% DOPA + 0 1% aminocaproic acid in DW) These are corn pared with the negative control group (no adhesives used group) and the positive control group (Tissucol Duo Quick group) The adult male Sprague Dawley it model was adopted and the hemostatic adhesion activities of each group were assessed by the adhesion strength test and the morphologic features of adhesion The liver tissues wet e used Histologic assessment of adhesion was accessed using light microscopy Results Bleeding was controlled immediately after application of Bleestop A and partial adhesion was ob served after 30 s More than 95% area of the resected surface attached after 1 min In Bleestop B, partial adhesion was observed after 30 s More than 95% area of the resected surface was attached after 45 s Histologic evaluation showed that Bleestop A and B mediated organized adhesion rapidly between both resected tissue surfaces The adhesion strength of Bleestop A and B was better than the negative control group and showed same adhesion strength as the positive control group after 3 min Conclusion Bleestop A and B showed significant hemostasis and adhesion capability within 1 min They are comparable to the positive control group (C) 2010 Elsevier Inc All rights reserved