Ki-67 expression in mature B-cell neoplasms: a flow cytometry study

被引：6

作者：

Marcondes, Natalia ^{[1
,2
]}

Fernandes, Flavo ^{[2
]}

Faulhaber, Gustavo ^{[1
,2
,3
]}

机构：

[1] Univ Fed Rio Grande do Sul, Programa Posgrad Med Ciencias Med, Porto Alegre, RS, Brazil

[2] Lab Zanol, Porto Alegre, RS, Brazil

[3] Univ Fed Rio Grande do Sul, Dept Med Interna, Porto Alegre, RS, Brazil

来源：

REVISTA DA ASSOCIACAO MEDICA BRASILEIRA | 2018年 / 64卷 / 06期

关键词：

Flow cytometry; Immunophenotyping; Ki-67; Antigen; CHRONIC LYMPHOCYTIC-LEUKEMIA; PROLIFERATIVE ACTIVITY; CYCLE; LYMPHOMA; ANTIGEN; MARKER;

D O I：

10.1590/1806-9282.64.06.525

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

OBJECTIVE: Ki-67 is a nuclear protein associated with cellular proliferation in normal or leukemic conditions that can help identify more aggressive diseases and is usually evaluated with immunohistochemistry. The aim of this was to assess Ki-67 expression on mature B-cell neoplasms samples with flow cytometry immunophenotyping. METHOD: After surface staining with CD19 and CD45, intracellular staining for Ki-67 was performed in leukemic mature B-cells. Ki-67 expression was evaluated with flow cytometry. RESULTS: Ki-67 expression was higher in mantle cell lymphoma, Burkitt lymphoma, and diffuse large B-cell lymphoma cases. It was also associated with CD38 mean fluorescence intensity. CONCLUSIONS: Ki-67 expression evaluated by flow cytometry can be a useful tool in the diagnosis of mature B-cell neoplasms. More studies are needed to validate Ki-67 assessment with flow cytometry immunophenotyping.

引用

页码：525 / 529

页数：5

共 30 条

[1]

AYDIN A, 1995, INT J ONCOL, V7, P1433

[2] B-cell chronic lymphocytic leukaemia cells show specific changes in membrane protein expression during different stages of cell cycle [J].

Bennett, Fiona ;

Rawstron, Andy ;

Plummer, Marieth ;

de Tute, Ruth ;

Moreton, Paul ;

Jack, Andrew ;

Hillmen, Peter .

BRITISH JOURNAL OF HAEMATOLOGY, 2007, 139 (04) :600-604

[3]

BRONS PPT, 1992, BLOOD, V80, P2336

[4] Intraclonal Complexity in Chronic Lymphocytic Leukemia: Fractions Enriched in Recently Born/Divided and Older/Quiescent Cells [J].

Calissano, Carlo ;

Damle, Rajendra N. ;

Marsilio, Sonia ;

Yan, Xiao-Jie ;

Yancopoulos, Sophia ;

Hayes, Gregory ;

Emson, Claire ;

Murphy, Elizabeth J. ;

Hellerstein, Marc K. ;

Sison, Cristina ;

Kaufman, Matthew S. ;

Kolitz, Jonathan E. ;

Allen, Steven L. ;

Rai, Kanti R. ;

Ivanovic, Ivana ;

Dozmorov, Igor M. ;

Roa, Sergio ;

Scharff, Matthew D. ;

Li, Wentian ;

Chiorazzi, Nicholas .

MOLECULAR MEDICINE, 2011, 17 (11-12) :1374-1382

[5] Proliferative response of mantle cell lymphoma cells stimulated by CD40 ligation and IL-4 [J].

Castillo, R ;

Mascarenhas, J ;

Telford, W ;

Chadburn, A ;

Friedman, SM ;

Schattner, EJ .

LEUKEMIA, 2000, 14 (02) :292-298

[6]

Chen Wen-Jie, 2015, Asian Pac J Cancer Prev, V16, P411

[7] BTK inhibition targets in vivo CLL proliferation through its effects on B-cell receptor signaling activity [J].

Cheng, S. ;

Ma, J. ;

Guo, A. ;

Lu, P. ;

Leonard, J. P. ;

Coleman, M. ;

Liu, M. ;

Buggy, J. J. ;

Furman, R. R. ;

Wang, Y. L. .

LEUKEMIA, 2014, 28 (03) :649-657

[8] CD38 expression labels an activated subset within chronic lymphocytic leukemia clones enriched in proliferating B cells [J].

Damle, Rajendra N. ;

Tembumi, Sonal ;

Calissano, Carlo ;

Yancopoulos, Sophia ;

Banapour, Taraneh ;

Sison, Cristina ;

Allen, Steven L. ;

Rai, Kanti R. ;

Chiorazzi, Nicholas .

BLOOD, 2007, 110 (09) :3352-3359

[9] Validation of Cell-based Fluorescence Assays: Practice Guidelines from the ICSH and ICCS - Part II - Preanalytical Issues [J].

Davis, Bruce H. ;

Dasgupta, Amar ;

Kussick, Steven ;

Han, Jin-Yeong ;

Estrellado, Annalee .

CYTOMETRY PART B-CLINICAL CYTOMETRY, 2013, 84 (05) :286-290

[10] Flow cytometry and its use in the diagnosis and management of mature lymphoid malignancies [J].

de Tute, Ruth M. .

HISTOPATHOLOGY, 2011, 58 (01) :90-105

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