DNA Amplifier-Functionalized Metal-Organic Frameworks for Multiplexed Detection and Imaging of Intracellular mRNA

被引：63
作者：
Meng, Hong-Min [2 ]
Sho, Xinxin [2 ]
Chen, Juan [2 ]
Gao, Yuhui [2 ]
Qu, Lingbo [2 ]
Zhang, Ke [2 ]
Zhang, Xiao-Bing [1 ]
Li, Zhaohui [2 ]
机构：
[1] Hunan Univ, Coll Chem & Chem Engn, Changsha 410082, Peoples R China
[2] Zhengzhou Univ, Coll Chem, Henan Joint Int Res Lab Green Construct Funct Mol, Zhengzhou 450001, Peoples R China
来源：
ACS SENSORS | 2020年 / 5卷 / 01期
基金：
中国国家自然科学基金; 中国博士后科学基金;
关键词：
DNA amplifier; fluorescent probes; intracellular imaging; metal-organic frameworks; mRNA; NANOFLARES; MICRORNA;
D O I：
10.1021/acssensors.9b01759
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
DNA amplification is a useful technique for low-abundance biomarker detection and environmental monitoring because of its high signal-amplifying ability. However, intracellular application of DNA amplifiers remains challenging due to poor delivery efficiency and stability. Herein, we report an entropy-driven DNA amplifier-functionalized metal-organic framework (DNA amplifier-MOF) for the detection and imaging of multiple intracellular messenger RNAs (mRNAs). The DNA amplifier- MOF conjugate exhibits high cellular uptake, enhanced enzymatic stability, and good biocompatibility. Importantly, in the presence of phosphate ions, a surface-functionalized DNA amplifier can be released in cells with high efficiency, which facilitates the imaging of mRNA. This method is rapid and of high sensitivity/specificity, as validated in HepG2 and HL7702 cells for the imaging of TK1 and survivin mRNA, respectively. With further optimization, the strategy can become a powerful biotechnology tool for the detection of cancers at early stages and for elucidating biological processes.
引用
收藏
页码：103 / 109
页数：13
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