c-maf oncogene dysregulation in multiple myeloma: Frequency and biological relevance

被引:19
作者
Rasmussen, T [1 ]
Knudsen, LM
Dahl, IMS
Johnsen, HE
机构
[1] Univ Copenhagen, Herlev Hosp, Dept Haematol L 54P4, DK-2730 Herlev, Denmark
[2] Univ Tromso Hosp, Sect Haematol, N-9038 Tromso, Norway
关键词
multiple myeloma; c-maf; II-4; real-time PCR;
D O I
10.1080/1042819031000111035
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
To investigate the frequency and possible biological consequences of c-maf dysregulation, we designed c-maf and IL-4 real-time RT-PCR assays for determination of c-maf and IL-4 mRNA levels. Using the c-maf real-time RT-PCR assay, we tested a panel of 14 B-cell lines, 135 diagnostic bone marrow (BM) samples from patients with multiple myeloma and 10 BM samples from normal donors. In B cell lines and flowsorted CD38(++)/CD19(-)/CD56(++) myeloma plasma cells (N = 14) the c-maf/GAPDH and IL-4/GAPDH ratios were determined simultaneously using real time RT-PCR. All B cell lines used in the study were characterized by flow cytometry and tested for the presence of Ebstein-Barr virus (EBV). B-cell lines, that were PCR negative for EBV and had a phenotype typical for primary myeloma cells, expressed medium to high levels of c-maf mRNA. However, all EBV PCR positive cell lines, showed a more immature phenotype, lacked expression of aberrant surface markers and contained very low levels of c-maf mRNA. In 4.4% (6/135) of MM patients tested, a c-maf mRNA level comparable to the cell line RPMI 8226 containing at (16:22), translocation was found. In addition, all c-maf positive myeloma cell lines and CD38(++)/CD19(-)/CD56(++) myeloma plasma cells tested were IL-4 negative. In conclusion, high levels of c-maf mRNA were observed in "true MM cell lines" and 4.4% of MM patients. Further, c-maf dysregulation in myeloma plasma cells did not cause induction of IL-4 transcription.
引用
收藏
页码:1761 / 1766
页数:6
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