Cadmium toxicity investigated at the physiological and biophysical levels under environmentally relevant conditions using the aquatic model plant Ceratophyllum demersum

被引:73
作者
Andresen, Elisa [1 ,2 ]
Kappel, Sophie [2 ]
Staerk, Hans-Joachim [3 ]
Riegger, Ulrike [2 ]
Borovec, Jakub [4 ,5 ]
Mattusch, Juergen [3 ]
Heinz, Andrea [6 ]
Schmelzer, Christian E. H. [6 ]
Matouskova, Sarka [7 ]
Dickinson, Bryan [8 ]
Kuepper, Hendrik [1 ,2 ,9 ]
机构
[1] CAS, Ctr Biol, Inst Plant Mol Biol, Dept Plant Biophys & Biochem, Branisovska 31-1160, CZ-37005 Ceske Budejovice, Czech Republic
[2] Univ Konstanz, Dept Biol, D-78457 Constance, Germany
[3] UFZ Helmholtz Ctr Environm Res, Dept Analyt Chem, Permoserstr 15, D-04318 Leipzig, Germany
[4] CAS, Ctr Biol, Inst Hydrobiol, Dept Hydrochem & Ecosyst Modelling, Sadkach 7, CZ-37005 Ceske Budejovice, Czech Republic
[5] Univ South Bohemia, Fac Sci, Dept Ecosyst Biol, Branisovska 1760, CZ-37005 Ceske Budejovice, Czech Republic
[6] Univ Halle Wittenberg, Inst Pharm, Wolfgang Langenbeck Str 4, D-06120 Halle, Saale, Germany
[7] CAS, Inst Geol, Rozvojova 269, CZ-16500 Prague 6, Czech Republic
[8] Univ Chicago, Dept Chem, GCIS E 319A,929 E 57th St, Chicago, IL 60637 USA
[9] Univ South Bohemia, Fac Biol Sci, Branisovska 31-1160, CZ-37005 Ceske Budejovice, Czech Republic
关键词
Cadmium (Cd); Ceratophyllum demersum; environmentally relevant; light-harvesting complexes (LHCs); macrophyte; toxic metals; toxicity; METAL-SUBSTITUTED CHLOROPHYLLS; LIGHT-HARVESTING COMPLEX; INDUCED OXIDATIVE STRESS; PHOTOSYSTEM-II; ELECTRON-TRANSPORT; INDUCED INHIBITION; NITRIC-OXIDE; OXYGEN; COPPER; ACCUMULATION;
D O I
10.1111/nph.13840
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Cadmium (Cd) is an important environmental pollutant and is poisonous to most organisms. We aimed to unravel the mechanisms of Cd toxicity in the model water plant Ceratophyllum demersum exposed to low (nM) concentrations of Cd as are present in nature. Experiments were conducted under environmentally relevant conditions, including nature-like light and temperature cycles, and a low biomass to water ratio. We measured chlorophyll (Chl) fluorescence kinetics, oxygen exchange, the concentrations of reactive oxygen species and pigments, metal binding to proteins, and the accumulation of starch and metals. The inhibition threshold concentration for most parameters was 20 nM. Below this concentration, hardly any stress symptoms were observed. The first site of inhibition was photosynthetic light reactions (the maximal quantum yield of photosystem II (PSII) reaction centre measured as F-v/F-m, light-acclimated PSII activity Phi(PSII), and total Chl). Trimers of the PSII light-harvesting complexes (LHCIIs) decreased more than LHC monomers and detection of Cd in the monomers suggested replacement of magnesium (Mg) by Cd in the Chl molecules. As a consequence of dysfunctional photosynthesis and energy dissipation, reactive oxygen species (superoxide and hydrogen peroxide) appeared. Cadmium had negative effects on macrophytes at much lower concentrations than reported previously, emphasizing the importance of studies applying environmentally relevant conditions. A chain of inhibition events could be established.
引用
收藏
页码:1244 / 1258
页数:15
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