Microrobotic assembly of shape-controllable microstructures to perfusable 3D cell-laden microtissues

被引：0

作者：

Cui, Juan ^{[1
]}

Wang, Huaping ^{[1
]}

Shi, Qing ^{[1
]}

Li, Jianing ^{[1
]}

Zheng, Zhiqiang ^{[1
]}

Sun, Tao ^{[1
]}

Huang, Qiang ^{[1
]}

Fukuda, Toshio ^{[1
]}

机构：

[1] Beijing Inst Technol, Sch Mechatron Engn, Beijing, Peoples R China

来源：

2017 IEEE 7TH ANNUAL INTERNATIONAL CONFERENCE ON CYBER TECHNOLOGY IN AUTOMATION, CONTROL, AND INTELLIGENT SYSTEMS (CYBER) | 2017年

基金：

中国国家自然科学基金; 北京市自然科学基金;

关键词：

3D assembly; PEGDA; microrobotic system; long-term culture; TISSUE CONSTRUCTS; FABRICATION; HYDROGELS;

D O I：

暂无

中图分类号：

TP [自动化技术、计算机技术];

学科分类号：

0812 ;

摘要：

Tissue engineered cellular microtissues by three-dimensional assembly to clearly model human organs in vitro is increasingly needed. In this paper, cell-encapsulated 3D microstructures with micro vessel-like lumen were fabricated using PEGDA (poly (ethylene) glycol diacrylate) hydrogels. The available DMD-based (Digital Mirror Device) photolithography system was utilized to fabricate 2D cellular micromodules in a microfluidic channel with no need of mask. The heterogeneous micromodules were assembled by a modified rail-guided microrobotic system w ith a straight forward strategy and high efficiency. The adjustment to align assembled micromodules to be a regular geometry relied on liquid surface tension. With rapid 2D fabrication and 3D assembly, NIH/3T3 cells encapsulated in hydrogels can be survived for over 14 days in 2D micromodules and 2days in 3D microstructures with incubation. Long-term culture of cells were performed with different concentration of PEGDA, and shown that cells viability increased by decreasing PEGDA concentration. Cell adhesion was improved by adding RGDS (Arg-Gly-Asp-Ser) peptide sequence with observation under optical microscope. The assembled 3D cellular microtissues have a significant potential to be used in regenerative medicine in the future.

引用

页码：13 / 18

页数：6

共 22 条

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