β-Carotene and astaxanthin with human and bovine serum albumins

被引：103

作者：

Li, Xiangrong ^{[1
,2
]}

Wang, Gongke ^{[2
]}

Chen, Dejun ^{[2
]}

Lu, Yan ^{[2
]}

机构：

[1] Xinxiang Med Univ, Sch Basic Med, Dept Chem, Xinxiang 453003, Henan, Peoples R China

[2] Henan Normal Univ, Sch Chem & Chem Engn, Key Lab Green Chem Media & React, Minist Educ, Xinxiang 453007, Henan, Peoples R China

来源：

FOOD CHEMISTRY | 2015年 / 179卷

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

beta-Carotene; Astaxanthin; Human serum albumin; Bovine serum albumin; Fluorescence spectroscopy; UV-vis absorption spectroscopy; FT-IR; FLUORESCENCE SPECTROSCOPY; BINDING; SURFACTANTS; BERBERINE; BEHAVIOR;

D O I：

10.1016/j.foodchem.2015.01.133

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

beta-Carotene and astaxanthin are two carotenoids with powerful antioxidant properties. In this study, the interaction of these two carotenoids with human serum albumin (HSA) and bovine serum albumin (BSA) under physiological conditions was investigated using several spectroscopic techniques. The experimental results indicate the quenching mechanism of HSA/BSA, by the two carotenoids, is a static process. The binding constants and number of binding sites were evaluated at different temperatures. Thermodynamic investigations revealed the interaction between the two carotenoids and HSA/BSA is synergistically driven by enthalpy and entropy, and hydrophobic forces and electrostatic attraction have a significant role in the reactions. Binding site I was found to be the primary binding site for beta-carotene and astaxanthin. In addition, as shown by synchronous fluorescence spectroscopy and FT-IR, the two carotenoids may induce conformational and micro-environmental changes in HSA/BSA. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：213 / 221

页数：9

共 34 条

[1] Characterization of subdomain IIA binding site of human serum albumin in its native, unfolded, and refolded states using small molecular probes [J].

Abou-Zied, Osama K. ;

Al-Shihi, Othman I. K. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (32) :10793-10801

[2] Structure and properties of carotenoids in relation to function [J].

Britton, G .

FASEB JOURNAL, 1995, 9 (15) :1551-1558

[3] Direct Observation of the β-Carotene Reaction with Hydroxyl Radical [J].

Chen, Chang-Hui ;

Han, Rui-Min ;

Liang, Ran ;

Fu, Li-Min ;

Wang, Peng ;

Ai, Xi-Cheng ;

Zhang, Jian-Ping ;

Skibsted, Leif H. .

JOURNAL OF PHYSICAL CHEMISTRY B, 2011, 115 (09) :2082-2089

[4] Studies on the interaction between scopoletin and two serum albumins by spectroscopic methods [J].

Cheng, Zhengjun .

JOURNAL OF LUMINESCENCE, 2012, 132 (10) :2719-2729

[5] Interaction of bovine (BSA) and human (HSA) serum albumins with ionic surfactants: spectroscopy and modelling [J].

Gelamo, EL ;

Silva, CHTP ;

Imasato, H ;

Tabak, M .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2002, 1594 (01) :84-99

[6] Interaction of pyrene-end-capped poly(ethylene oxide) with bovine serum albumin and human serum albumin in aqueous buffer medium: A fluorometric study [J].

Haldar, B ;

Mallick, A ;

Chattopadhyay, N .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2005, 80 (03) :217-224

[7] Automated Ligand- and Structure-Based Protocol for in Silico Prediction of Human Serum Albumin Binding [J].

Hall, Michelle Lynn ;

Jorgensen, William L. ;

Whitehead, Lewis .

JOURNAL OF CHEMICAL INFORMATION AND MODELING, 2013, 53 (04) :907-922

[8] ATOMIC-STRUCTURE AND CHEMISTRY OF HUMAN SERUM-ALBUMIN [J].

HE, XM ;

CARTER, DC .

NATURE, 1992, 358 (6383) :209-215

[9] Investigation of the Interaction between Berberine and Human Serum Albumin [J].

Hu, Yan-Jun ;

Liu, Yi ;

Xiao, Xiao-He .

BIOMACROMOLECULES, 2009, 10 (03) :517-521

[10] Astaxanthin, a carotenoid with potential in human health and nutrition [J].

Hussein, G ;

Sankawa, U ;

Goto, H ;

Matsumoto, K ;

Watanabe, H .

JOURNAL OF NATURAL PRODUCTS, 2006, 69 (03) :443-449

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