Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies

被引：397
|
作者：
Fairfoul, Graham [1 ]
McGuire, Lynne I. [1 ]
Pal, Suvankar [1 ,2 ]
Ironside, James W. [1 ]
Neumann, Juliane [3 ]
Christie, Sharon [4 ]
Joachim, Catherine [4 ]
Esiri, Margaret [4 ]
Evetts, Samuel G. [3 ]
Rolinski, Michal [3 ]
Baig, Fahd [3 ]
Ruffmann, Claudio [3 ]
Wade-Martins, Richard [5 ]
Hu, Michele T. M. [3 ]
Parkkinen, Laura [3 ]
Green, Alison J. E. [1 ]
机构：
[1] Univ Edinburgh, Western Gen Hosp, Natl CJD Res & Surveillance Unit, Edinburgh EH4 2XU, Midlothian, Scotland
[2] Western Gen Hosp, Ctr Clin Brain Sci, Edinburgh EH4 2XU, Midlothian, Scotland
[3] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Neurosci, Oxford Parkinsons Dis Ctr, Level 6 West Wing, Oxford OX3 9DU, England
[4] Univ Oxford, John Radcliffe Hosp, Oxford Project Investigate Memory & Ageing OPTIMA, Nuffield Dept Clin Neurosci, Level 6 West Wing, Oxford OX3 9DU, England
[5] Univ Oxford, Oxford Parkinsons Dis Ctr, Dept Physiol Anat & Genet, Le Gros Clark Bldg,South Parks Rd, Oxford OX1 3QX, England
来源：
ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY | 2016年 / 3卷 / 10期
基金：
英国医学研究理事会;
关键词：
SLEEP BEHAVIOR DISORDER; PARKINSONS-DISEASE; CEREBROSPINAL-FLUID; BIOMARKERS; COHORT;
D O I：
10.1002/acn3.338
中图分类号：
R74 [神经病学与精神病学];
学科分类号：
摘要：
We have developed a novel real-time quaking-induced conversion RT-QuIC-based assay to detect alpha-synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha-synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and with an overall specificity of 100% when compared to Alzheimer and control cerebrospinal fluid. Patients with neuropathologically confirmed tauopathies (progressive supranuclear palsy; corticobasal degeneration) gave negative results. These results suggest that RT-QuiC analysis of cerebrospinal fluid is potentially useful for the early clinical assessment of patients with alpha-synucleinopathies.
引用
收藏
页码：812 / 818
页数：7
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