Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies

被引：420

作者：

Fairfoul, Graham ^{[1
]}

McGuire, Lynne I. ^{[1
]}

Pal, Suvankar ^{[1
,2
]}

Ironside, James W. ^{[1
]}

Neumann, Juliane ^{[3
]}

Christie, Sharon ^{[4
]}

Joachim, Catherine ^{[4
]}

Esiri, Margaret ^{[4
]}

Evetts, Samuel G. ^{[3
]}

Rolinski, Michal ^{[3
]}

Baig, Fahd ^{[3
]}

Ruffmann, Claudio ^{[3
]}

Wade-Martins, Richard ^{[5
]}

Hu, Michele T. M. ^{[3
]}

Parkkinen, Laura ^{[3
]}

Green, Alison J. E. ^{[1
]}

机构：

[1] Univ Edinburgh, Western Gen Hosp, Natl CJD Res & Surveillance Unit, Edinburgh EH4 2XU, Midlothian, Scotland

[2] Western Gen Hosp, Ctr Clin Brain Sci, Edinburgh EH4 2XU, Midlothian, Scotland

[3] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Neurosci, Oxford Parkinsons Dis Ctr, Level 6 West Wing, Oxford OX3 9DU, England

[4] Univ Oxford, John Radcliffe Hosp, Oxford Project Investigate Memory & Ageing OPTIMA, Nuffield Dept Clin Neurosci, Level 6 West Wing, Oxford OX3 9DU, England

[5] Univ Oxford, Oxford Parkinsons Dis Ctr, Dept Physiol Anat & Genet, Le Gros Clark Bldg,South Parks Rd, Oxford OX1 3QX, England

来源：

ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY | 2016年 / 3卷 / 10期

基金：

英国医学研究理事会;

关键词：

SLEEP BEHAVIOR DISORDER; PARKINSONS-DISEASE; CEREBROSPINAL-FLUID; BIOMARKERS; COHORT;

D O I：

10.1002/acn3.338

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

We have developed a novel real-time quaking-induced conversion RT-QuIC-based assay to detect alpha-synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha-synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and with an overall specificity of 100% when compared to Alzheimer and control cerebrospinal fluid. Patients with neuropathologically confirmed tauopathies (progressive supranuclear palsy; corticobasal degeneration) gave negative results. These results suggest that RT-QuiC analysis of cerebrospinal fluid is potentially useful for the early clinical assessment of patients with alpha-synucleinopathies.

引用

页码：812 / 818

页数：7

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