Molecular forms and fragments of salivary MMP-8 in relation to periodontitis

被引:34
作者
Gursoy, Ulvi K. [1 ]
Kononen, Eija [1 ,2 ]
Tervahartiala, Taina [3 ]
Gursoy, Mervi [1 ]
Pitkanen, Jari [3 ]
Torvi, Paula [3 ]
Suominen, Anna Liisa [4 ,5 ,6 ]
Pussinen, Pirkko [3 ]
Sorsa, Timo [3 ,7 ]
机构
[1] Univ Turku, Inst Dent, Periodontol, Turku, Finland
[2] Oral Hlth Care, Welf Div, Turku, Finland
[3] Univ Helsinki, Helsinki Univ Hosp, Dept Oral & Maxillofacial Dis, Helsinki, Finland
[4] Univ Eastern Finland, Inst Dent, Kuopio, Finland
[5] Natl Inst Hlth & Welf, Hlth Monitoring Unit, Helsinki, Finland
[6] Kuopio Univ Hosp, Dept Oral & Maxillofacial Dis, Kuopio, Finland
[7] Karolinska Inst, Dept Dent Med, Periodontol, Huddinge, Sweden
基金
芬兰科学院;
关键词
collagenase; neutrophil; periodontal disease; saliva; HUMAN NEUTROPHIL COLLAGENASE; GINGIVAL CREVICULAR FLUID; LATENT HUMAN NEUTROPHIL; MATRIX METALLOPROTEINASES; PORPHYROMONAS-GINGIVALIS; ACTIVATION; RELEASE; EXPRESSION; PROTEASES; MARKERS;
D O I
10.1111/jcpe.13024
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Aim To investigate the molecular forms of salivary matrix metalloproteinase (MMP)-8 in relation to periodontitis. Materials and Methods Molecular forms, degree of activation and fragmentation of neutrophilic and mesenchymal-type MMP-8 isoforms were analysed from salivary samples of 81 subjects with generalized periodontitis, 63 subjects with localized periodontitis and 79 subjects without pocket teeth, by using western-immunoblots with computer quantitation. In addition, human recombinant proMMP-8 was in vitro activated by Treponema denticola chymotrypsin-like protease (Td-CTLP), sodium hypochlorite (NaOCl, 1 mM, oxidant) or amino phenyl mercuric acetate (APMA, 1 mM). Results In saliva of periodontitis-affected individuals, MMP-8 is found in multiple forms, that is, complexes, active and pro-forms of neutrophilic and mesenchymal-type MMP-8, and especially 20-27 kDa fragments. The quantity of these fragments was elevated in both localized and generalized forms of periodontitis. Moreover, the tested activators (Td-CTLP, NaOCl and APMA) activated inactive proMMP-8, resulting in fragments of 20-27 kDa, in vitro, and salivary concentrations of T. denticola correlated significantly with salivary levels of fragmented MMP-8. Conclusion The present results indicate that during the development and progression of periodontitis, MMP-8 appears as activated and fragmented, and treponemal proteases most likely play role in this cascade.
引用
收藏
页码:1421 / 1428
页数:8
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