DNA vaccines with single-chain Fv fused to fragment C of tetanus toxin induce protective immunity against lymphoma and myeloma

被引：222
|
作者：
King, CA [1 ]
Spellerberg, MB [1 ]
Zhu, DL [1 ]
Rice, J [1 ]
Sahota, SS [1 ]
Thompsett, AR [1 ]
Hamblin, TJ [1 ]
Radl, J [1 ]
Stevenson, FK [1 ]
机构：
[1] Southampton Univ Hosp Trust, Tenovus Lab, Mol Immunol Grp, Southampton SO16 6YD, Hants, England
来源：
NATURE MEDICINE | 1998年 / 4卷 / 11期
关键词：
D O I：
10.1038/3266
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Vaccination with idiotypic protein protects against B-cell lymphoma, mainly through anti-idiotypic antibody. For use in patients, DNA vaccines containing single-chain Fv derived from tumor provide a convenient alternative vaccine delivery system. However, single-chain Fv sequence alone induces low anti-idiotypic response and poor protection against lymphoma. Fusion of the gene encoding fragment C of tetanus toxin to single-chain Fv substantially promotes the anti-idiotypic response and induces strong protection against B-cell lymphoma. The same fusion design also induces protective immunity against a surface Ig-negative myeloma. These findings indicate that fusion to a pathogen sequence allows a tumor antigen to engage diverse immune mechanisms that suppress growth. This fusion design has the added advantage of overcoming potential tolerance to tumor that may exist in patients.
引用
收藏
页码：1281 / 1286
页数：6
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