Characterization of Genes Encoding Virulence Determinants and Toxins in Staphylococcus aureus from Bovine Milk in Central Italy

被引：17

作者：

Casagrande Proietti, Patrizia ^{[1
]}

Coppola, Giacomo ^{[2
]}

Bietta, Annalisa ^{[1
]}

Marenzoni, Maria Luisa ^{[2
]}

Hyatt, Doreene R. ^{[3
]}

Coletti, Mauro ^{[2
]}

Passamonti, Fabrizio ^{[2
]}

机构：

[1] Univ Perugia, Dept Biopathol Sci & Hyg Food & Anim Prod, Fac Vet Med, I-06126 Perugia, Italy

[2] Univ Perugia, Pathol Diagnost & Vet Clin Dept, Sport Horse Res Ctr, I-06126 Perugia, Italy

[3] Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2010年 / 72卷 / 11期

关键词：

bovine; PCR; staphylococcal enterotoxins; Staphylococcus aureus; virulence determinants; POLYMERASE-CHAIN-REACTION; PROTEIN-A GENE; INTRAMAMMARY INFECTIONS; SUBCLINICAL MASTITIS; SHOCK-SYNDROME; ENTEROTOXIN-C; STRAINS; COWS; CLUSTER; GOATS;

D O I：

10.1292/jvms.10-0158

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The aim of this study was to evaluate the genotypic characteristics of Staphylococcus amens isolates (n=170) from bovine milk collected from seven dairy farms in Italy. On the basis of cultural and biochemical properties and by amplification of the 23S rRNA specific to S. aureus, all isolates were identified as S. aureus. To genotypically characterize S. aurens isolates, genes encoding virulence determinants (one, clfA, spa-IgG-binding spa-X-region, fnbA and fnbB, cap5 and cap8) and staphylococcal enterotoxins (sea, seb, sec, sed, see, seg, seh, sei, sej) were investigated using a PCR technique. The results showed that the isolates of S. aureus in each farm had the same genotypic characteristics, while the isolates genotipically differed between the different farms. The present study might help to understand the distribution of prevalent S. aureus strains in dairy farms.

引用

页码：1443 / 1448

页数：6

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