Kinetic resolution of (R/S)-propranolol (1-isopropylamino-3-(1-naphtoxy)-2-propanolol) catalyzed by immobilized preparations of Candida antarctica lipase B (CAL-B)

被引：34

作者：

Barbosa, Oveimar ^{[1
]}

Ariza, Cesar ^{[1
]}

Ortiz, Claudia ^{[2
,3
]}

Torres, Rodrigo ^{[1
]}

机构：

[1] Univ Ind Santander, Escuela Quim, GIBIP, Bucaramanga, Colombia

[2] Univ Ind Santander, Escuela Bacteriol, Bucaramanga, Colombia

[3] Univ Ind Santander, Clin Lab, Bucaramanga, Colombia

来源：

NEW BIOTECHNOLOGY | 2010年 / 27卷 / 06期

关键词：

ENZYMATIC RESOLUTION; INTERFACIAL ACTIVATION; HUMAN-PLASMA; ENANTIOSELECTIVITY; ENANTIOMERS; SUPPORTS; ENZYMES; MODULATION; ADSORPTION; SEPARATION;

D O I：

10.1016/j.nbt.2010.07.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Enzyme preparations of Candida antarctica B lipase (CAL-B) - immobilized on Eupergit C and partially modified Eupergit C supports - were tested for kinetic resolution of (R/S)-propranolol, using vinyl acetate as acyl donor, and toluene as organic solvent The effects of (R/S)-propranolol concentration, vinyl acetate concentration and biocatalyst loading on the esterification and resolution of propranolol were studied Additionally, different types of immobilized lipase derivatives were also evaluated in terms of its selectivity on kinetic resolution of (R,S)-propranolol These derivatives showed different enantiomeric ratios (E), with high enantiomeric ratios (E = 57) with CAL-B immobilized on Eupergit C supports

引用

页码：844 / 850

页数：7

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