A multiplex real-time PCR method to detect and quantify mitochondrial DNA deletions in individual cells

被引：74
作者：
Krishnan, Kim J. [1 ]
Bender, Andreas
Taylor, Robert W.
Turnbull, Douglass M.
机构：
[1] Univ Newcastle, Sch Med, Sch Neurol Neurobiol & Psychiat, Mitochondrial Res Grp, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England
[2] Univ Newcastle, Inst Ageing & Hlth, Newcastle Upon Tyne NE4 6BE, Tyne & Wear, England
[3] Univ Munich, Dept Neurol, D-81377 Munich, Germany
[4] Univ Newcastle, Inst Human Genet, Newcastle Upon Tyne NE1 3BZ, Tyne & Wear, England
来源：
ANALYTICAL BIOCHEMISTRY | 2007年 / 370卷 / 01期
基金：
英国惠康基金; 英国医学研究理事会;
关键词：
D O I：
10.1016/j.ab.2007.06.024
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
[No abstract available]
引用
收藏
页码：127 / 129
页数：3
相关论文
共 14 条
[1]   High levels of mitochondrial DNA deletions in substantia nigra neurons in aging and Parkinson disease [J].
Bender, A ;
Krishnan, KJ ;
Morris, CM ;
Taylor, GA ;
Reeve, AK ;
Perry, RH ;
Jaros, E ;
Hersheson, JS ;
Betts, J ;
Klopstock, T ;
Taylor, RW ;
Turnbull, DM .
NATURE GENETICS, 2006, 38 (05) :515-517
[2]   Mitochondrial DNA deletion in "identical'' twin brothers [J].
Blakely, EL ;
He, L ;
Taylor, RW ;
Chinnery, PF ;
Lightowlers, RN ;
Schaefer, AM ;
Turnbull, DM .
JOURNAL OF MEDICAL GENETICS, 2004, 41 (02) :e19
[3]   Quantification of mitochondrial DNA deletion, depletion, and overreplication: Application to diagnosis [J].
Chabi, B ;
de Camaret, BM ;
Duborjal, H ;
Issartel, JP ;
Stepien, G .
CLINICAL CHEMISTRY, 2003, 49 (08) :1309-1317
[4]   ASSOCIATION OF MITOCHONDRIAL-DNA DAMAGE WITH AGING AND CORONARY ATHEROSCLEROTIC HEART-DISEASE [J].
CORRALDEBRINSKI, M ;
SHOFFNER, JM ;
LOTT, MT ;
WALLACE, DC .
MUTATION RESEARCH, 1992, 275 (3-6) :169-180
[5]   Mitochondrial DNA copy number threshold in mtDNA depletion myopathy [J].
Durham, SE ;
Bonilla, E ;
Samuels, DC ;
DiMauro, S ;
Chinnery, PF .
NEUROLOGY, 2005, 65 (03) :453-455
[6]   Real-time PCR analysis of a 3895 bp mitochondrial DNA deletion in nonmelanoma skin cancer and its use as a quantitative marker for sunlight exposure in human skin [J].
Harbottle, A. ;
Birch-Machin, M. A. .
BRITISH JOURNAL OF CANCER, 2006, 94 (12) :1887-1893
[7]   Detection and quantification of mitochondrial DNA deletions in individual cells by real-time PCR [J].
He, LP ;
Chinnery, PF ;
Durham, SE ;
Blakely, EL ;
Wardell, TM ;
Borthwick, GM ;
Taylor, RW ;
Turnbull, DM .
NUCLEIC ACIDS RESEARCH, 2002, 30 (14) :e68
[8]   Mitochondrial DNA polymerase-γ and human disease [J].
Hudson, Gavin ;
Chinnery, Patrick F. .
HUMAN MOLECULAR GENETICS, 2006, 15 :R244-R252
[9]   Mitochondrial DNA deletions are abundant and cause functional impairment in aged human substantia nigra neurons [J].
Kraytsberg, Y ;
Kudryavtseva, E ;
Mckee, AC ;
Geula, C ;
Kowall, NW ;
Khrapko, K .
NATURE GENETICS, 2006, 38 (05) :518-520
[10]   Single-molecule PCR: an artifact-free PCR approach for the analysis of somatic mutations [J].
Kraytsberg, Y ;
Khrapko, K .
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2005, 5 (05) :809-815
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