The neuron-astrocyte-microglia triad in CA3 after chronic cerebral hypoperfusion in the rat: Protective effect of dipyridamole

被引：31

作者：

Lana, Daniele ^{[1
]}

Ugolini, Filippo ^{[1
]}

Melani, Alessia ^{[2
]}

Nosi, Daniele ^{[3
]}

Pedata, Felicita ^{[2
]}

Giovannini, Maria Grazia ^{[1
]}

机构：

[1] Univ Florence, Sect Pharmacol & Clin Oncol, Dept Hlth Sci, Viale Pieraccini 6, I-50139 Florence, Italy

[2] Univ Florence, Sect Pharmacol & Toxicol, Dept NEUROFARBA, Viale Pieraccini 6, I-50139 Florence, Italy

[3] Univ Florence, Dept Expt & Clin Med, I-50139 Florence, Italy

来源：

EXPERIMENTAL GERONTOLOGY | 2017年 / 96卷

关键词：

Apoptosis; Phagocytosis; Phagoptosis; CA3; Confocal microscopy; Neuron-astrocyte-microglia triad; CAROTID-ARTERY OCCLUSION; ENDOTHELIAL GROWTH-FACTOR; MESSENGER-RNA EXPRESSION; REACTIVE ASTROCYTES; BLOOD-FLOW; SELECTIVE VULNERABILITY; INTRACRANIAL BYPASS; EATEN ALIVE; IN-VITRO; BRAIN;

D O I：

10.1016/j.exger.2017.06.006

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

We investigated the quantitative and morphofunctional alterations of neuron-astrocyte-microglia triads in CA3 hippocampus, in comparison to CA1, after 2 Vessel Occlusion (2VO) and the protective effect of dipyridamole. We evaluated 3 experimental groups: sham-operated rats (sham, n = 15), 2VO-operated rats treated with vehicle (2VO-vehicle, n = 15), and 2VO-operated rats treated with dipyridamole from day 0 to day 7 (2VO-dipyridamole, n = 15), 90 days after 2VO. We analyzed Stratum Pyramidalis (SP), Stratum Lucidum (SL) and Stratum Radiatum (SR) of CA3. 1) ectopic neurons increased in SL and SR of 2VO-vehicle, and 2VO-dipyridamole rats; 2) apoptotic neurons increased in SP of 2VO-vehicle rats and dipyridamole reverted this effect; 3) astrocytes increased in SP, SL and SR of 2VO-vehicle and 2VO-dipyridamole rats; 4) TNF-alpha expression increased in astrocytes, blocked by dipyridamole, and in dendrites in SR of 2VO-vehicle rats; 5) total microglia increased in SL and SR of 2VO-vehicle and 2VO-dipyridamole rats; 6) triads increased in SR of 2VO-vehicle rats and dipyridamole reverted this effect. Microglia cooperated with astrocytes to phagocytosis of apoptotic neurons and debris, and engulfed ectopic non-fragmented neurons in SL of 2VO-vehicle and 2VO-dipyridamole rats, through a new mechanism called phagoptosis. CA3 showed a better adaptive capacity than CA1 to the ischemic insult, possibly due to the different behaviour of astrocytes and microglial cells. Dipyridamole had neuroprotective effects. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：46 / 62

页数：17

共 107 条

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