Elevated expression of IL-18 but not IL-1β gene is associated with NALP3 and AIM2 inflammasome in Polycystic Ovary Syndrome

被引:20
|
作者
Rostamtabar, Maryam [1 ]
Esmaeilzadeh, Sedighe [2 ]
Karkhah, Ahmad [1 ,3 ]
Amiri, Mania [2 ]
Rahmani, Abolfazl [1 ]
Bakouei, Fatemeh [2 ]
Nouri, Hamid Reza [2 ,3 ,4 ]
机构
[1] Babol Univ Med Sci, Student Res Comm, Babol Sar, Iran
[2] Babol Univ Med Sci, Hlth Res Inst, Infertil & Reprod Hlth Res Ctr, Babol Sar, Iran
[3] Babol Univ Med Sci, Hlth Res Inst, Cellular & Mol Biol Res Ctr, Babol Sar, Iran
[4] Babol Univ Med Sci, Hlth Res Inst, Immunoregulat Res Ctr, Babol Sar, Iran
关键词
IL-1; beta; IL-18; Inflammasome; Polycystic Ovary Syndrome; INSULIN-RESISTANCE; MESSENGER-RNA; INTERLEUKIN-1-BETA; RECEPTOR; PROTEIN; PROFILE; WOMEN;
D O I
10.1016/j.gene.2020.144352
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Inflammasome complex mediated interleukin 1 beta (IL-1 beta) and interleukin 18 (IL-18) production may be involved in immunopathogenesis of polycystic ovary syndrome (PCOS). Therefore, this study was conducted to investigate involved inflammasome pathways in PCOS. Therefore, inflammasome genes expression and serum level of IL-1 beta were evaluated in 30 patients with confirmed PCOS and 30 women without PCOS. A remarkable increase in expression of the nucleotide binding and oligomerization domain (NOD)-like receptor (NLR) family pyrin domain-containing 3 (NALP3), absent in melanoma 2 (AIM2), IL-18 and associated speck-like protein containing a caspase recruitment domain (CARD); (ASC) genes in PCOS were observed (p < 0.05). In contrast, expression level of NALP1, NALP12, NLR family apoptosis inhibitory proteins (NAIP), NLR family caspase recruitment domain (CARD) domain containing 4 (NLRC4) and IL-1 beta genes was not significant. Although the IL-1 beta protein level in serum of COS patients with BMI >= 25 was significantly higher than PCOS patient with BMI < 25, but there was no significant difference in non-PCOS individuals with BMI < 25 or >= 25. Furthermore, significant correlation between expression of AIM2 (r = 0.83, p = 0.032) and NALP3 (r = 0.59, p = 0.0001) was observed with IL-18, while a positive correlation (r = 0.84, p = 0.0001) was revealed between NAIP and 1L-1 beta. Based on the obtained results on inflammasome components along with increased expression of IL-10 especially in overweight patients, it can be concluded that IL-18 expression as well as IL-1 beta is probably due to activation of AIM2, NALP3 or NAIP inflammasome, which may play a critical role in immunopathology of PCOS.
引用
收藏
页数:7
相关论文
共 50 条
  • [11] AIM2 Mediates Inflammation-Associated Renal Damage in Hepatitis B Virus-Associated Glomerulonephritis by Regulating Caspase-1, IL-1β, and IL-18
    Zhen, Junhui
    Zhang, Le
    Pan, Jiachao
    Ma, Shumin
    Yu, Xiaojian
    Li, Xiaobo
    Chen, Shijun
    Du, Wenjun
    MEDIATORS OF INFLAMMATION, 2014, 2014
  • [12] Distinct Licensing of IL-18 and IL-1β Secretion in Response to NLRP3 Inflammasome Activation
    Schmidt, Rebecca L.
    Lenz, Laurel L.
    PLOS ONE, 2012, 7 (09):
  • [13] Activation of the NALP3 inflammasome in dendritic cells induces IL-1\#946;-dependent adaptive immunity against tumors
    Tesniere, Antoine
    Ghiringhelli, Francois
    Apetoh, Lionel
    Zitvogel, Laurence
    Kroemer, Guido
    CANCER RESEARCH, 2009, 69
  • [14] Author Correction: AIM2 inflammasome-derived IL-1β induces postoperative ileus in mice
    Kristof Johannes Hupa
    Kathy Stein
    Reiner Schneider
    Mariola Lysson
    Bianca Schneiker
    Veit Hornung
    Eicke Latz
    Yoichiro Iwakura
    Jörg C. Kalff
    Sven Wehner
    Scientific Reports, 10
  • [15] IL-1 beta but not the NALP3 inflammasome is an important determinant of endothelial cell responses to necrotic/dangerous trophoblastic debris
    Wei, J.
    Chen, Q.
    James, J. L.
    Stone, P. R.
    Chamley, L. W.
    PLACENTA, 2015, 36 (12) : 1385 - 1392
  • [16] Macrophage IL-1β contributes to tumorigenesis through paracrine AIM2 inflammasome activation in the tumor microenvironment
    Chew, Zhi Huan
    Cui, Jianzhou
    Sachaphibulkij, Karishma
    Tan, Isabelle
    Kar, Shreya
    Koh, Kai Kiat
    Singh, Kritika
    Lim, Hong Meng
    Lee, Soo Chin
    Kumar, Alan Prem
    Gasser, Stephan
    Lim, Lina H. K.
    FRONTIERS IN IMMUNOLOGY, 2023, 14
  • [17] An association of IL-18 gene polymorphisms with impaired glucose regulation in Korean patients with polycystic ovary syndrome
    Kim, J. W.
    Yoon, T. K.
    Lee, W. S.
    Han, J. E.
    Lyu, S. W.
    Shim, S. H.
    HUMAN REPRODUCTION, 2011, 26 : I329 - I329
  • [18] Correlation between IL-1 β, IL-1Ra gene polymorphism and occurrence of polycystic ovary syndrome infertility
    Xia, Yu-Hong
    Yao, Li
    Zhang, Zhan-Xin
    ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE, 2013, 6 (03) : 232 - 236
  • [19] Correlation between IL-1β,IL-1Ra gene polymorphism and occurrence of polycystic ovary syndrome infertility
    Yu-Hong Xia
    Li Yao
    Zhan-Xin Zhang
    Asian Pacific Journal of Tropical Medicine, 2013, (03) : 232 - 236
  • [20] IL-1 receptor antagonist levels are associated with glucose tolerance in polycystic ovary syndrome
    Luotola, Kari
    Piltonen, Terhi T.
    Puurunen, Johanna
    Tapanainen, Juha S.
    CLINICAL ENDOCRINOLOGY, 2016, 85 (03) : 430 - 435