Identification of outer membrane proteins of Vibrio parahaemolyticus and Vibrio alginolyticus altered in response to γ-irradiation or long-term starvation

被引：19

作者：

Ben Abdallah, Fethi ^{[1
,2
]}

Ellafi, Ali ^{[1
]}

Lagha, Rihab ^{[1
]}

Bakhrouf, Amina ^{[1
]}

Namane, Abdelkader ^{[3
]}

Rousselle, Jean-Claude ^{[3
]}

Lenormand, Pascal ^{[3
]}

Kallel, Hela ^{[2
]}

机构：

[1] Fac Pharm, Lab Anal Traitement & Valorisat Polluants Environ, Monastir 5000, Tunisia

[2] Inst Pasteur Tunis, Unite Fermentat & Dev Vaccins Virol, Tunis 1002, Tunisia

[3] Inst Pasteur, CNRS, URA 2185, F-75724 Paris 15, France

来源：

RESEARCH IN MICROBIOLOGY | 2010年 / 161卷 / 10期

关键词：

Vibrio; Starvation; gamma-Irradiation; Alteration; Outer membrane proteins; Mass spectrometry; PROTEOMIC ANALYSIS; EXPRESSION; LIPOPOLYSACCHARIDE; TEMPERATURE; RESISTANCE; RADIATION; OMPW;

D O I：

10.1016/j.resmic.2010.10.009

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Vibrio parahaemolyticus and Vibrio alginolyticus were subjected to gamma-irradiation (0.5 kGy) or starvation by incubation for 8 months in seawater to study modifications in their outer membrane protein patterns. After treatment, outer membrane protein profiles of starved or gamma-irradiated bacteria were found to be altered when analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Altered proteins were identified by mass spectrometry (MS and MS/MS) and analyses revealed that OmpU can be considered a starvation stress-induced protein. In addition, expression of OtnA, OmpW, OmpA and peptidoglycan-associated lipoprotein decreased to non-detectable levels in starved cells. Furthermore. MltA-interacting protein MipA appeared under gamma-irradiation or starvation conditions. Thus, it can be considered to be a gamma-irradiation, long-term starvation stress protein in some vibrios. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

引用

页码：869 / 875

页数：7

共 38 条

[1]

Andrews L., 2003, Journal of Aquatic Food Product Technology, V12, P71, DOI 10.1300/J030v12n03_07

[2] Protective role of L-tyrosine in the sterilization of ceruloplasmin therapeutic protein by gamma-irradiation [J].

Assemand, E ;

Lacroix, M ;

Mateescu, MA .

RADIATION PHYSICS AND CHEMISTRY, 2004, 71 (1-2) :405-409

[3]

Ben Abdallah F, 2009, ANN MICROBIOL, V59, P63

[4]

Ben Kahla NA, 2008, MICROBIOL RES, V163, P323

[5] Effect of gamma radiation on heat shock protein expression of four foodborne pathogens [J].

Caillet, S. ;

Millette, M. ;

Dussault, D. ;

Shareck, F. ;

Lacroix, M. .

JOURNAL OF APPLIED MICROBIOLOGY, 2008, 105 (05) :1384-1391

[6] Effects of environmental pH on membrane proteins in Borrelia burgdorferi [J].

Carroll, JA ;

Garon, CF ;

Schwan, TG .

INFECTION AND IMMUNITY, 1999, 67 (07) :3181-3187

[7]

CONTRERAS I, 1995, FEMS MICROBIOL LETT, V133, P105, DOI 10.1111/j.1574-6968.1995.tb07869.x

[8] Global analysis of outer membrane proteins from Leptospira interrogans serovar Lai [J].

Cullen, PA ;

Cordwell, SJ ;

Bulach, DM ;

Haake, DA ;

Adler, B .

INFECTION AND IMMUNITY, 2002, 70 (05) :2311-2318

[9] Expression of acid-stable proteins and modified lipopolysaccharide of Yersinia pestis in acidic growth medium [J].

Feodorova, VA ;

Devidariani, ZL .

JOURNAL OF MEDICAL MICROBIOLOGY, 2001, 50 (11) :979-985

[10]

FilaliMouhim A, 1997, INT J RADIAT BIOL, V72, P63, DOI 10.1080/095530097143545

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